{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T07:19:53Z","timestamp":1766647193867,"version":"3.41.2"},"reference-count":85,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T00:00:00Z","timestamp":1447718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","award":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"],"award-info":[{"award-number":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001871","name":"Foundation for Science and Technology","doi-asserted-by":"crossref","award":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"],"award-info":[{"award-number":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001871","name":"Foundation for Science and Technology","doi-asserted-by":"crossref","award":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"],"award-info":[{"award-number":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"],"award-info":[{"award-number":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"],"award-info":[{"award-number":["PEst-OE\/SAU\/UI4013\/2011","UID\/AMB\/50017\/2013.","SFRH\/BPD\/48853\/2008","FRH\/BPD\/74868\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV\u2010B\u2010irradiated skin cells. Cells were exposed for 24\u2009h to 10\u2009M lycopene, and subsequently irradiated and left to recover for another 24\u2009h period. Thereafter, several parameters were analyzed by FCM and RT\u2010PCR: genotoxicity\/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin\u2010V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of <jats:italic>BAX<\/jats:italic> gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S\u2010phase transition and consequent decrease of cells in G0\/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.<\/jats:p>","DOI":"10.1155\/2016\/8214631","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T21:02:19Z","timestamp":1447794139000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["The Effect of Lycopene Preexposure on UV\u2010B\u2010Irradiated Human Keratinocytes"],"prefix":"10.1155","volume":"2016","author":[{"given":"Andreia","family":"Ascenso","sequence":"first","affiliation":[]},{"given":"Tiago","family":"Pedrosa","sequence":"additional","affiliation":[]},{"given":"S\u00f3nia","family":"Pinho","sequence":"additional","affiliation":[]},{"given":"Francisco","family":"Pinho","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Miguel P. Ferreira de","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Helena","family":"Cabral Marques","sequence":"additional","affiliation":[]},{"given":"Helena","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Sandra","family":"Sim\u00f5es","sequence":"additional","affiliation":[]},{"given":"Concei\u00e7\u00e3o","family":"Santos","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2015,11,17]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.5507\/bp.2003.019"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/b505312a"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2006.00466.x"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700510"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0250"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1081296"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2008.00120.x"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-007-0051-z"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/125.7.1854"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2007.02.015"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476910802482854"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232574399"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781444317633.ch3"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1473-2130.2004.00075.x"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.435"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1207\/s15327914nc4702_11"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.2.287"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-4165(00)00014-3"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0891-5849(02)00831-6"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-999-0454-9"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.1998.1.39"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1207\/s15327914nc3602_7"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/10715760000301321"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2005.09.004"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.2012.666968"},{"key":"e_1_2_8_26_2","first-page":"28","article-title":"Topical delivery of oily actives using solid lipid particles","volume":"19","author":"Souto E. B.","year":"2007","journal-title":"Pharmaceutical Technology Europe"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507691752"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-004-0536-5"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520160304"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520200103"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041094"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.9.2119"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.25.1600"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2005.01.004"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/01635589709514521"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-214-44105"},{"key":"e_1_2_8_37_2","first-page":"55","volume-title":"Methods in Enzymology","author":"Cooney R. V.","year":"1993"},{"key":"e_1_2_8_38_2","doi-asserted-by":"publisher","DOI":"10.1080\/01635589809514640"},{"key":"e_1_2_8_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.05.016"},{"key":"e_1_2_8_40_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.761"},{"key":"e_1_2_8_41_2","first-page":"413","article-title":"Protective effects of resveratrol on UVB-irradiated HaCaT cells through attenuation of the caspase pathway","volume":"19","author":"Park K.","year":"2008","journal-title":"Oncology Reports"},{"key":"e_1_2_8_42_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83317-0"},{"key":"e_1_2_8_43_2","first-page":"537","article-title":"Photosensitizer effect of curcumin on UVB-irradiated HaCaT cells through activation of caspase pathways","volume":"17","author":"Park K.","year":"2007","journal-title":"Oncology Reports"},{"key":"e_1_2_8_44_2","doi-asserted-by":"publisher","DOI":"10.1039\/b503397g"},{"key":"e_1_2_8_45_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3436"},{"key":"e_1_2_8_46_2","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2014.864777"},{"key":"e_1_2_8_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092980"},{"key":"e_1_2_8_48_2","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers","volume":"132","author":"Rozen S.","year":"2000","journal-title":"Methods in Molecular Biology"},{"volume-title":"UCSC in Silico PCR","year":"2013","author":"Kent J.","key":"e_1_2_8_49_2"},{"key":"e_1_2_8_50_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2005.12.1047"},{"key":"e_1_2_8_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_8_52_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0525"},{"key":"e_1_2_8_53_2","first-page":"30","article-title":"Supplementation with tomato juice increases plasma lycopene but does not alter susceptibility to oxidation of low-density lipoproteins from renal transplant recipients","volume":"52","author":"Sutherland W. H. F.","year":"1999","journal-title":"Clinical Nephrology"},{"key":"e_1_2_8_54_2","doi-asserted-by":"publisher","DOI":"10.1177\/153537020623100313"},{"key":"e_1_2_8_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(03)00235-7"},{"key":"e_1_2_8_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf000503o"},{"key":"e_1_2_8_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.1998.tb01762.x"},{"key":"e_1_2_8_58_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf803747j"},{"key":"e_1_2_8_59_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408390802565889"},{"key":"e_1_2_8_60_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867033457557"},{"key":"e_1_2_8_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2005.12.009"},{"key":"e_1_2_8_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.12.3754"},{"key":"e_1_2_8_63_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/22.4.573"},{"key":"e_1_2_8_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204707"},{"key":"e_1_2_8_65_2","doi-asserted-by":"publisher","DOI":"10.1667\/0033-7587(2000)154[0151:KETURR]2.0.CO;2"},{"key":"e_1_2_8_66_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-006-0595-x"},{"key":"e_1_2_8_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2004.11"},{"key":"e_1_2_8_68_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12010.x"},{"key":"e_1_2_8_69_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700008"},{"key":"e_1_2_8_70_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202x.2004.23514.x"},{"key":"e_1_2_8_71_2","doi-asserted-by":"publisher","DOI":"10.1208\/pt060243"},{"key":"e_1_2_8_72_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10847-008-9456-y"},{"key":"e_1_2_8_73_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2011.01038.x"},{"key":"e_1_2_8_74_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-06-3350"},{"key":"e_1_2_8_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2009.12.013"},{"key":"e_1_2_8_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1011-1344(01)00217-2"},{"key":"e_1_2_8_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1011-1344(00)00149-4"},{"key":"e_1_2_8_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8734(92)90039-r"},{"key":"e_1_2_8_79_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.21670"},{"volume-title":"Composition Having Tanning and Photoprotective Activity, and Its Cosmetic Applications","year":"2000","author":"Harang B.","key":"e_1_2_8_80_2"},{"key":"e_1_2_8_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.1.237S"},{"key":"e_1_2_8_82_2","doi-asserted-by":"publisher","DOI":"10.1177\/153537020523000303"},{"key":"e_1_2_8_83_2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot078311"},{"key":"e_1_2_8_84_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086092"},{"key":"e_1_2_8_85_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.249"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2016\/8214631.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2016\/8214631.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2016\/8214631","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T01:48:58Z","timestamp":1722908938000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2016\/8214631"}},"subtitle":[],"editor":[{"given":"Luciano","family":"Saso","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2015,11,17]]},"references-count":85,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1155\/2016\/8214631"],"URL":"https:\/\/doi.org\/10.1155\/2016\/8214631","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"type":"print","value":"1942-0900"},{"type":"electronic","value":"1942-0994"}],"subject":[],"published":{"date-parts":[[2015,11,17]]},"assertion":[{"value":"2015-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8214631"}}