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strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by<jats:italic>Cupriavidus necator<\/jats:italic>DSM 428 with used cooking oil (UCO) as the sole carbon source. With the exponential feeding strategy, a cell dry mass of 21.3\u2009\u00b1\u20090.9\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>was obtained, with a polymer content of 84.0\u2009\u00b1\u20094.5\u2009wt.%, giving an overall volumetric productivity of 4.5\u2009\u00b1\u20090.2\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>\u00a0day<jats:sup>\u22121<\/jats:sup>. However, the highest P(3HB) volumetric productivity, 12.6\u2009\u00b1\u20090.8\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>\u00a0day<jats:sup>\u22121<\/jats:sup>, was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8\u2009\u00b1\u20090.6\u2009g\u2009L<jats:sup>\u22121<\/jats:sup>). The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>to 2.6\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>\u2009g\u2009mol<jats:sup>\u22121<\/jats:sup>, with low polydispersity indexes of 1.2-1.6. Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174\u00b0C and 3.0-4.0\u00b0C, respectively. The polymer exhibited a crystallinity ranging from 52 to 65%. The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production. 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