{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T05:52:15Z","timestamp":1775627535866,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"vor","delay-in-days":201,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["AGR\/UID00239\/2013"],"award-info":[{"award-number":["AGR\/UID00239\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. Acetylcholinesterase (AChE) inhibitors are used to treat Alzheimer\u2019s patients because they enhance cholinergic neurotransmission. It is urgent to find new and efficient inhibitors from natural sources, highly bioavailable with low or no toxicity. The plant kingdom is extremely rich in a variety of compounds that are potent AChE inhibitors: flavonoids and other phenolic compounds have been recognized as promising Alzheimer\u2019s treatment agents. In this study, <jats:italic>in vitro<\/jats:italic> acetylcholinesterase inhibition, antioxidant activities, and total flavonoid and phenolic contents of ethanol\u2010water extracts from <jats:italic>Quercus suber<\/jats:italic> cork and corkback were evaluated. <jats:italic>Methods<\/jats:italic>. The acetylcholinesterase activity was determined by a colorimetric assay based on Ellman\u2019s methodology. The Folin\u2013Ciocalteu colorimetric method was used for total phenolic content determination and the aluminium chloride method for the determination of total flavonoid content. Antioxidant activity assays were performed using the DPPH and FRAP assays. <jats:italic>Results<\/jats:italic>. The acetylcholinesterase inhibitory activity from <jats:italic>Q. suber<\/jats:italic> cork and corkback ethanol\u2010water extracts was as follows: 62% inhibition with corkback extracts over 0.5\u2009mg\/mL and around 49% inhibition in cork extracts over 1.0\u2009mg\/mL extracts\u2019 concentration. Regarding the DPPH radical scavenging activity, the concentrations of cork and corkback ethanol\u2010water extracts required for 50% DPPH inhibition (IC50) were 3.2\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL and 4.0\u20135.2\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. Corkback extracts are less effective than Trolox standard (3.2\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) but cork extracts showed the same free radical scavenging activity compared to Trolox. Cork and corkback extracts have antioxidant power of 750.9\u2013775.4\u2009mg TEAC\/g extract and 1051.2\u20132052.4\u2009mg TEAC\/g extract, respectively, which are significantly higher than the ones obtained with Trolox: 19.6\u201321.0\u2009mg TEAC\/g extract (cork assays) and 57.4\u201366.3\u2009mg TEAC\/g extract (corkback assays). The amounts of total phenolic (TPC) and flavonoid (TFC) compounds were 8.7\u201332.3\u2009mg GAE\/g and 4.8\u201310.7\u2009mg CE\/g dry mass for cork and 5.4\u20135.7\u2009mg GAE\/g and 42.5\u2009mg CE\/g dry mass for corkback extracts, respectively, using catechin (CE) and GAE (gallic acid) as standards. <jats:italic>Conclusion<\/jats:italic>. These findings demonstrate the remarkable potential of these extracts as valuable source of antioxidants with interesting acetylcholinesterase inhibitory activity.<\/jats:p>","DOI":"10.1155\/2020\/3825629","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T23:32:08Z","timestamp":1595287928000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["<i>In Vitro<\/i> Screening for Acetylcholinesterase Inhibition and Antioxidant Activity of <i>Quercus suber<\/i> Cork and Corkback Extracts"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7637-3466","authenticated-orcid":false,"given":"Joana","family":"Ferreira","sequence":"first","affiliation":[]},{"given":"Sara","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Helena","family":"Pereira","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.000299"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/1570159x11311040004"},{"key":"e_1_2_9_3_2","first-page":"965","article-title":"Medicinal plants in therapy","volume":"63","author":"Farnsworth N. 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