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We have previously shown that ceramide production contributes to RCD induced by acetic acid and is involved in mitochondrial outer membrane permeabilization and cytochrome <jats:italic>c<\/jats:italic> release, especially through hydrolysis of complex sphingolipids catalyzed by Isc1p. Recently, we also showed that Sch9p regulates the translocation of Isc1p from the endoplasmic reticulum into mitochondria, perturbing sphingolipid balance and determining cell fate. In this study, we addressed the role of other signaling proteins in acetic acid-induced RCD. We found that single deletion of <jats:italic>PKH1<\/jats:italic> or <jats:italic>YPK1<\/jats:italic>, as shown for <jats:italic>SCH9<\/jats:italic> and <jats:italic>ISC1<\/jats:italic>, leads to an increase in cell survival in response to acetic acid and that Pkh1\/2p-dependent phosphorylation of Ypk1p and Sch9p increases under these conditions. These results indicate that Pkh1p regulates acetic acid-induced RCD through Ypk1p and Sch9p. In addition, our results suggest that Pkh1p-Ypk1p is necessary for <jats:italic>isc1\u0394<\/jats:italic> resistance to acetic acid-induced RCD. Moreover, double deletion of <jats:italic>ISC1<\/jats:italic> and <jats:italic>PKH1<\/jats:italic> has a drastic effect on cell survival associated with increased ROS accumulation and release of cytochrome <jats:italic>c<\/jats:italic>, which is counteracted by overexpression of the PKA pathway negative regulator <jats:italic>PDE2<\/jats:italic>. Overall, our results suggest that Pkh1p-Ypk1p and Pkh1p-Sch9p pathways contribute to RCD induced by acetic acid.<\/jats:p>","DOI":"10.1155\/2020\/7095078","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T23:30:45Z","timestamp":1585870245000},"page":"1-14","source":"Crossref","is-referenced-by-count":12,"title":["Pkh1p-Ypk1p and Pkh1p-Sch9p Pathways Are Activated by Acetic Acid to Induce a Mitochondrial-Dependent Regulated Cell Death"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2849-4017","authenticated-orcid":true,"given":"Ant\u00f3nio","family":"Rego","sequence":"first","affiliation":[{"name":"Departamento de Biologia, Centro de Biologia Molecular e Ambiental, Universidade do Minho, Braga, Portugal"},{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, Porto, 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