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The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clinic. This study is aimed to generating molecular subtypes of gastric cancer using techniques available in routine diagnostic practice in a series of Moroccan gastric cancer patients. In addition, we assessed the associations between molecular subtypes, clinicopathological features, and prognosis. Methods. Ninety-seven gastric cancer cases were classified according to TCGA, ACRG, and integrated classifications using a panel of four molecular markers (EBV, MSI, E-cadherin, and p53). HER2 status and PD-L1 expression were also evaluated. These markers were analyzed using immunohistochemistry (E-cadherin, p53, HER2, and PD-L1), in situ hybridization (EBV and HER2 equivocal cases), and multiplex PCR (MSI). Results. Our results showed that the subtypes presented distinct clinicopathological features and prognosis. EBV-positive gastric cancers were found exclusively in male patients. The GS (TCGA classification), MSS\/EMT (ACRG classification), and E-cadherin aberrant subtype (integrated classification) presented the Lauren diffuse histology enrichment and tended to be diagnosed at a younger age. The MSI subtype was associated with a better overall survival across all classifications (TCGA, ACRG, and integrated classification). The worst prognosis was observed in the EBV subtype (TCGA and integrated classification) and MSS\/EMT subtype (ACRG classification). Discussion\/Conclusion. We reported a reproducible and affordable gastric cancer subtyping algorithms that can reproduce the recently recognized TCGA, ACRG, and integrated gastric cancer classifications, using techniques available in routine diagnosis. These simplified classifications can be employed not only for molecular classification but also in predicting the prognosis of gastric cancer patients.<\/jats:p>","DOI":"10.1155\/2021\/9980410","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T22:20:07Z","timestamp":1627597207000},"page":"1-12","source":"Crossref","is-referenced-by-count":37,"title":["Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0986-4588","authenticated-orcid":true,"given":"Jean Paul","family":"Nshizirungu","sequence":"first","affiliation":[{"name":"Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6405-8322","authenticated-orcid":true,"given":"Sanae","family":"Bennis","sequence":"additional","affiliation":[{"name":"Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco"}]},{"given":"Ihsane","family":"Mellouki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Faculty of Medicine and Pharmacy, Abdelmalek Essaadi University, Tangier, Morocco"}]},{"given":"Mohammed","family":"Sekal","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology, Clinical Research and Public Health, Faculty of Medicine and Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco"}]},{"given":"Dafr-Allah","family":"Benajah","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hassan II University Hospital, Fez, Morocco"}]},{"given":"Nada","family":"Lahmidani","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hassan II University Hospital, Fez, Morocco"}]},{"given":"Hicham","family":"El Bouhaddouti","sequence":"additional","affiliation":[{"name":"Department of Visceral Surgery, Hassan II University Hospital, Fez, Morocco"}]},{"given":"Karim","family":"Ibn Majdoub","sequence":"additional","affiliation":[{"name":"Department of Visceral Surgery, Hassan II University Hospital, Fez, Morocco"}]},{"given":"Sidi Adil","family":"Ibrahimi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hassan II University Hospital, Fez, Morocco"}]},{"given":"S\u00f3nia Pires","family":"Celeiro","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B\u2019s - PT Government Associate Laboratory, Braga\/Guimar\u00e3es, Portugal"}]},{"given":"Marta","family":"Viana-Pereira","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B\u2019s - PT Government Associate Laboratory, Braga\/Guimar\u00e3es, Portugal"}]},{"given":"Fernanda Franco","family":"Munari","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, S\u00e3o Paulo, Brazil"}]},{"given":"Guilherme Gomes","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Pathology Department, Barretos Cancer Hospital, Barretos, S\u00e3o Paulo, Brazil"}]},{"given":"Vinicius","family":"Duval","sequence":"additional","affiliation":[{"name":"Pathology Department, Barretos Cancer Hospital, Barretos, S\u00e3o Paulo, Brazil"}]},{"given":"Iara","family":"Santana","sequence":"additional","affiliation":[{"name":"Pathology Department, Barretos Cancer Hospital, Barretos, S\u00e3o Paulo, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9639-7940","authenticated-orcid":true,"given":"Rui Manuel","family":"Reis","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal"},{"name":"ICVS\/3B\u2019s - 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