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Utilization of faecal markers may allow us to fill this gap, accommodating animal welfare and intensive labour issues that are major limitations of traditional techniques. This study aimed to evaluate the potential of n-alkane markers (C25 to C33) to estimate diet composition, feed intake its digestibility on three captive maleBison bonasus fed on known amounts of straw, and a beeswax labelled concentrate feed. Feeds and faecal samples were taken daily during 10 days. Prior to calculations, faecal concentrations were corrected for incomplete faecal recovery (FR). Results indicated that 4\u20135 days were sufficient for these markers to reach a steady concentration in bison faeces. Accurate estimates of diet composition and feed intake were obtained not differing from known values. Results suggest that faecal recovery of n-alkanes in bison are incomplete and tend to increase with carbon-chain length. Apparent dry-matter digestibility (DMDap) estimates were affected by the n-alkane (C27, C29,C31, and C33) used in the calculations. Estimates of DMDap obtained with application of FR corrections were 6.3% higher than those without correction. Results indicate that feeding a known amount of beeswax labelled supplement can be successfully used to estimate composition, feed intake, and its digestibility, requiring the application of n-alkane FR data.<\/jats:p>","DOI":"10.1155\/2022\/9800265","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T17:50:06Z","timestamp":1667238606000},"page":"1-9","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of N-alkanes as Faecal Markers to Estimate Diet Composition, Feed Intake, and Digestibility in European Bison (Bison bonasus)"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3341-861X","authenticated-orcid":true,"given":"Luis Miguel","family":"Mendes Ferreira","sequence":"first","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD-CITAB), Inov4Agro (Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production), Vila Real, Portugal"}]},{"given":"Daniela","family":"Pereira","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Center, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD-CECAV), Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2219-7898","authenticated-orcid":true,"given":"Ana","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Center, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD-CECAV), Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0049-6424","authenticated-orcid":true,"given":"Miguel","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD-CITAB), Inov4Agro (Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production), Vila Real, Portugal"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s13364-013-0129-x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2020.108500"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.2307\/3898308"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1590\/s0103-90162007000600015"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1071\/an15153"},{"key":"6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.5772\/67345","article-title":"Utilization of biomarkers to study the grazing behavior of herbivore species","volume-title":"Herbivores","author":"L. 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