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We used metagenomic sequencing of cell\u2010free DNA (cfDNA) in hedgehog blood to identify possible causes of HDD. We detected a novel circovirus species in the European hedgehog, providing the first record of a circovirus within the mammalian order Eulipotyphla. The novel circovirus genome exhibits the characteristic circovirus structure, including a functional replicase (REP) and capsid (CAP) gene. Phylogenetic analysis placed all four detected genomes in a monophyletic clade, most closely related to sequences isolated from dogs. Subsequent PCR\u2010based screening of 188 hedgehog liver samples demonstrated a high prevalence (61%) of this circovirus in hedgehogs brought to wildlife rescue centers, however, without any significant association with HDD. Since circoviruses are well known to interfere with host immunity across mammalian and avian taxa, the high level of circovirus detection in hedgehogs warrants further research into the role of this novel virus in hedgehog health.<\/jats:p>","DOI":"10.1155\/2024\/4670252","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T08:48:56Z","timestamp":1732697336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High Prevalence of a Novel Circovirus in the European Hedgehog (<i>Erinaceus europaeus<\/i>), a Common Species in Decline"],"prefix":"10.1155","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6688-8848","authenticated-orcid":false,"given":"Kevin P.","family":"Mulder","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3160-503X","authenticated-orcid":false,"given":"Frank","family":"Pasmans","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8815-5485","authenticated-orcid":false,"given":"Filip van","family":"Nieuwerburgh","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1228-4664","authenticated-orcid":false,"given":"Naomi","family":"Terriere","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4372-700X","authenticated-orcid":false,"given":"Moira","family":"Kelly","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3965-0294","authenticated-orcid":false,"given":"Seline","family":"Bregman","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1388-4266","authenticated-orcid":false,"given":"Elin","family":"Verbrugghe","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7609-5649","authenticated-orcid":false,"given":"An","family":"Martel","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.01.011"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-012418-012751"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00329"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.01.006"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7426"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24033051"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.landurbplan.2011.05.010"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0248422"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41437-022-00567-5"},{"key":"e_1_2_13_10_2","unstructured":"ReeveN. 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