{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T09:48:52Z","timestamp":1761126532858},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,4]],"date-time":"2002-01-04T00:00:00Z","timestamp":1010102400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p><jats:italic>Objective:<\/jats:italic> To investigate if introital and vaginal flushing samples inoculated on chromogenic agar could increase\nthe recovery rate and rapid identification of <jats:italic>Candida<\/jats:italic> and non\u2010<jats:italic>albicans<\/jats:italic> species, as compared to culture of posterior\nvaginal fornix samples on Sabouraud agar and speciation of isolates by biochemical tests.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> Samples from the introitus and the posterior vaginal fornix and vaginal lavage samples were collected\nfrom 91 women with a history suggestive of recurrent vulvovaginal candidosis (RVVC), and with a suspected new\nattack of the condition. The specimens were cultured on Sabouraud and CHROMagar<jats:sup><jats:italic>\u00ae<\/jats:italic><\/jats:sup>\n. Speciation of yeast\nisolates was made on the chromogenic agar by API 32C<jats:sup><jats:italic>\u00ae<\/jats:italic><\/jats:sup>kits and by an atomized system (Vitek<jats:sup><jats:italic>\u00ae<\/jats:italic><\/jats:sup>).<\/jats:p><jats:p><jats:italic>Results:<\/jats:italic> Forty\u2010six (51%) women were positive for <jats:italic>Candida<\/jats:italic> fromone or more of the samples. The introital cultures\nwere positive in 43 (47%) women, both on Sabouraud and chromogenic agar. From the posterior vaginal fomix,\n42 (46%) women were positive on the Sabouraud and 43 (47%) on chromogenic agar cultures, while the vaginal\nlavage cultures yielded <jats:italic>Candida<\/jats:italic> on those two media in 40 (44%) and 4l (45%) cases, respectively. <jats:italic>Candida albicans<\/jats:italic>\nwas the most frequent species recovered, from 40 (87%) cases, followed by <jats:italic>C. krusei<\/jats:italic>  in 4 (9%), <jats:italic>C. glabrata<\/jats:italic> in\n2 (4%), and  <jats:italic>C. parapsilosis<\/jats:italic> in one case. There was only onewoman who had a mixed yeast infection, by <jats:italic>C. albicans<\/jats:italic>\nand <jats:italic>C. krusei<\/jats:italic> . There was only one discrepancy in the speciation as demonstrated by mean of chromogenic agar and\nAPI 32C kit.<\/jats:p><jats:p><jats:italic>Conclusions:<\/jats:italic> Neither cultures of introital nor of vaginal lavage samples increases the detection rate of <jats:italic>Candida<\/jats:italic> in\nRVVC cases as compared to cultures of posterior vaginal fornix samples. Use of chromogenic agar is a convenient\nand reliable means to detect colonization by <jats:italic>Candida<\/jats:italic> and differentiate between  <jats:italic>C. albicans<\/jats:italic> and non\u2010<jats:italic>albicans<\/jats:italic>\nspecies.<\/jats:p>","DOI":"10.1155\/s1064744902000078","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T13:12:46Z","timestamp":1173273166000},"page":"89-92","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Can the Diagnosis of Recurrent Vulvovaginal Candidosis BeImproved by Use of Vaginal Lavage Samples and Cultures onChromogenic Agar?"],"prefix":"10.1155","volume":"10","author":[{"given":"N.","family":"Novikova","sequence":"first","affiliation":[]},{"given":"A.","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"P.-A.","family":"M\u00e5rdh","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,1,4]]},"container-title":["Infectious Diseases in Obstetrics and Gynecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/idog\/2002\/602082.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S1064744902000078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T15:59:07Z","timestamp":1720022347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S1064744902000078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1155\/S1064744902000078"],"URL":"https:\/\/doi.org\/10.1155\/s1064744902000078","archive":["Portico"],"relation":{},"ISSN":["1064-7449"],"issn-type":[{"value":"1064-7449","type":"print"}],"subject":[],"published":{"date-parts":[[2002,1]]},"assertion":[{"value":"2001-10-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}