{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T14:14:15Z","timestamp":1793283255282,"version":"4.5.0"},"reference-count":68,"publisher":"American Association for Cancer Research (AACR)","issue":"1","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":82,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Cookies for Kid\u2019s Cancer","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]},{"name":"DIPG All-In","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]},{"DOI":"10.13039\/100007445","name":"Matthew Larson Foundation","doi-asserted-by":"crossref","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/100007445","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NCI Clinical Proteomics Tumor Analysis Consortium","award":["U01CA214114"],"award-info":[{"award-number":["U01CA214114"]}]},{"name":"NCI Academic Industrial Partnership","award":["R01CA235575"],"award-info":[{"award-number":["R01CA235575"]}]},{"name":"NCI Research Specialist Program","award":["R50CA211499"],"award-info":[{"award-number":["R50CA211499"]}]},{"DOI":"10.13039\/100001445","name":"Alex's Lemonade Stand Foundation for Childhood Cancer","doi-asserted-by":"crossref","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/100001445","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100015338","name":"Cancer Moonshot","doi-asserted-by":"crossref","award":["HHSN26100025"],"award-info":[{"award-number":["HHSN26100025"]}],"id":[{"id":"10.13039\/100015338","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"crossref","award":["U01TR002487"],"award-info":[{"award-number":["U01TR002487"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"crossref"}]},{"name":"St. Baldrick\u2019s Foundation","award":["SU2C-AACR-DT-27-17"],"award-info":[{"award-number":["SU2C-AACR-DT-27-17"]}],"id":[{"id":"https:\/\/ror.org\/03h1gea69","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["aacrjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Diffuse intrinsic pontine glioma (DIPG) remains a fatal brainstem tumor demanding innovative therapies. As B7-H3 (CD276) is expressed on central nervous system (CNS) tumors, we designed B7-H3\u2013specific chimeric antigen receptor (CAR) T cells, confirmed their preclinical efficacy, and opened BrainChild-03 (NCT04185038), a first-in-human phase I trial administering repeated locoregional B7-H3 CAR T cells to children with recurrent\/refractory CNS tumors and DIPG. Here, we report the results of the first three evaluable patients with DIPG (including two who enrolled after progression), who received 40 infusions with no dose-limiting toxicities. One patient had sustained clinical and radiographic improvement through 12 months on study. Patients exhibited correlative evidence of local immune activation and persistent cerebrospinal fluid (CSF) B7-H3 CAR T cells. Targeted mass spectrometry of CSF biospecimens revealed modulation of B7-H3 and critical immune analytes (CD14, CD163, CSF-1, CXCL13, and VCAM-1). Our data suggest the feasibility of repeated intracranial B7-H3 CAR T-cell dosing and that intracranial delivery may induce local immune activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance:<\/jats:title>\n                    <jats:p>This is the first report of repeatedly dosed intracranial B7-H3 CAR T cells for patients with DIPG and includes preliminary tolerability, the detection of CAR T cells in the CSF, CSF cytokine elevations supporting locoregional immune activation, and the feasibility of serial mass spectrometry from both serum and CSF.<\/jats:p>\n                    <jats:p>This article is highlighted in the In This Issue feature, p. 1<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1158\/2159-8290.cd-22-0750","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T10:01:07Z","timestamp":1666173667000},"page":"114-131","update-policy":"https:\/\/doi.org\/10.1158\/crossmark_policy","source":"Crossref","is-referenced-by-count":281,"title":["Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety"],"prefix":"10.1158","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3966-4985","authenticated-orcid":false,"given":"Nicholas A.","family":"Vitanza","sequence":"first","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-001X","authenticated-orcid":false,"given":"Ashley L.","family":"Wilson","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8948-7262","authenticated-orcid":false,"given":"Wenjun","family":"Huang","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8402-0900","authenticated-orcid":false,"given":"Kristy","family":"Seidel","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0834-4003","authenticated-orcid":false,"given":"Christopher","family":"Brown","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."},{"name":"4Therapeutic Cell Production Core, Seattle Children's Research Institute, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0735-7235","authenticated-orcid":false,"given":"Joshua A.","family":"Gustafson","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2520-8134","authenticated-orcid":false,"given":"Jason K.","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9967-8959","authenticated-orcid":false,"given":"Adam J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6491-1802","authenticated-orcid":false,"given":"Blake A.","family":"Baxter","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3673-1396","authenticated-orcid":false,"given":"Ryan W.","family":"Koning","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2387-108X","authenticated-orcid":false,"given":"Aquene N.","family":"Reid","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2710-606X","authenticated-orcid":false,"given":"Michael","family":"Meechan","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6152-3604","authenticated-orcid":false,"given":"Matthew C.","family":"Biery","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6638-3898","authenticated-orcid":false,"given":"Carrie","family":"Myers","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3733-0014","authenticated-orcid":false,"given":"Stephanie D.","family":"Rawlings-Rhea","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7610-9932","authenticated-orcid":false,"given":"Catherine M.","family":"Albert","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2376-9421","authenticated-orcid":false,"given":"Samuel R.","family":"Browd","sequence":"additional","affiliation":[{"name":"5Division of Neurosurgery, Seattle Children's Hospital and Department of Neurological Surgery, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2035-9876","authenticated-orcid":false,"given":"Jason S.","family":"Hauptman","sequence":"additional","affiliation":[{"name":"5Division of Neurosurgery, Seattle Children's Hospital and Department of Neurological Surgery, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6205-6614","authenticated-orcid":false,"given":"Amy","family":"Lee","sequence":"additional","affiliation":[{"name":"5Division of Neurosurgery, Seattle Children's Hospital and Department of Neurological Surgery, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7580-8934","authenticated-orcid":false,"given":"Jeffrey G.","family":"Ojemann","sequence":"additional","affiliation":[{"name":"5Division of Neurosurgery, Seattle Children's Hospital and Department of Neurological Surgery, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3382-6787","authenticated-orcid":false,"given":"Michael E.","family":"Berens","sequence":"additional","affiliation":[{"name":"6Cancer and Cell Biology Division, The Translational Genomics Research Institute (TGen), Phoenix, Arizona."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9063-5370","authenticated-orcid":false,"given":"Matthew D.","family":"Dun","sequence":"additional","affiliation":[{"name":"7School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, Callaghan, Australia."},{"name":"8Precision Medicine Research Program, Hunter Medical Research Institute, New Lambton Heights, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8001-5960","authenticated-orcid":false,"given":"Jessica B.","family":"Foster","sequence":"additional","affiliation":[{"name":"9Division of Oncology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania."},{"name":"10Department of Pediatrics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-695X","authenticated-orcid":false,"given":"Erin E.","family":"Crotty","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0225-6184","authenticated-orcid":false,"given":"Sarah E.S.","family":"Leary","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3471-1949","authenticated-orcid":false,"given":"Bonnie L.","family":"Cole","sequence":"additional","affiliation":[{"name":"11Department of Laboratories, Seattle Children's Hospital, Seattle, Washington."},{"name":"12Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8456-5209","authenticated-orcid":false,"given":"Francisco A.","family":"Perez","sequence":"additional","affiliation":[{"name":"13Department of Radiology, Seattle Children's Hospital, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6676-8253","authenticated-orcid":false,"given":"Jason N.","family":"Wright","sequence":"additional","affiliation":[{"name":"13Department of Radiology, Seattle Children's Hospital, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6310-3467","authenticated-orcid":false,"given":"Rimas J.","family":"Orentas","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3273-5549","authenticated-orcid":false,"given":"Tony","family":"Chour","sequence":"additional","affiliation":[{"name":"12Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington."},{"name":"14Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2889-243X","authenticated-orcid":false,"given":"Evan W.","family":"Newell","sequence":"additional","affiliation":[{"name":"12Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington."},{"name":"14Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5042-8580","authenticated-orcid":false,"given":"Jeffrey R.","family":"Whiteaker","sequence":"additional","affiliation":[{"name":"15Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8811-2124","authenticated-orcid":false,"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[{"name":"15Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6532-6499","authenticated-orcid":false,"given":"Amanda G.","family":"Paulovich","sequence":"additional","affiliation":[{"name":"15Clinical Research Division, Fred Hutchinson Cancer Center, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8775-6710","authenticated-orcid":false,"given":"Navin","family":"Pinto","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5679-1989","authenticated-orcid":false,"given":"Juliane","family":"Gust","sequence":"additional","affiliation":[{"name":"16Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"17Division of Pediatric Neurology, Department of Neurology, University of Washington, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1532-3200","authenticated-orcid":false,"given":"Rebecca A.","family":"Gardner","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."},{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7477-3063","authenticated-orcid":false,"given":"Michael C.","family":"Jensen","sequence":"additional","affiliation":[{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6777-9165","authenticated-orcid":false,"given":"Julie R.","family":"Park","sequence":"additional","affiliation":[{"name":"1Ben Towne Center for Childhood Cancer Research, Seattle Children's Research Institute, Seattle, Washington."},{"name":"2Department of Pediatrics, Seattle Children's Hospital, University of Washington, Seattle, Washington."},{"name":"3Seattle Children's Therapeutics, Seattle, Washington."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1086","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"2025121006581889200_bib1","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1093\/neuonc\/nox107","article-title":"Contemporary survival endpoints: an international diffuse intrinsic pontine glioma registry study","volume":"19","author":"Cooney","year":"2017","journal-title":"Neuro Oncol"},{"key":"2025121006581889200_bib2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s11940-019-0577-y","article-title":"Diffuse intrinsic pontine glioma: from diagnosis to next-generation clinical trials","volume":"21","author":"Vitanza","year":"2019","journal-title":"Curr Treat Options Neurol"},{"key":"2025121006581889200_bib3","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1007\/s00401-016-1545-1","article-title":"The 2016 World Health Organization classification of tumors of the central nervous system: a summary","volume":"131","author":"Louis","year":"2016","journal-title":"Acta Neuropathol"},{"key":"2025121006581889200_bib4","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1182\/blood-2017-02-769208","article-title":"Intent-to-treat leukemia remission by CD19 CAR T cells of defined formulation and dose in children and young adults","volume":"129","author":"Gardner","year":"2017","journal-title":"Blood"},{"key":"2025121006581889200_bib5","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1038\/s41586-022-04489-4","article-title":"GD2-CAR T cell therapy for H3K27M-mutated diffuse midline gliomas","volume":"603","author":"Majzner","year":"2022","journal-title":"Nature"},{"key":"2025121006581889200_bib6","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/j.1365-2559.2008.03070.x","article-title":"Small round blue cell tumours: diagnostic and prognostic usefulness of the expression of B7-H3 surface molecule","volume":"53","author":"Gregorio","year":"2008","journal-title":"Histopathology"},{"key":"2025121006581889200_bib7","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s11060-012-1021-2","article-title":"B7-H3, a potential therapeutic target, is expressed in diffuse intrinsic pontine glioma","volume":"111","author":"Zhou","year":"2013","journal-title":"J Neurooncol"},{"key":"2025121006581889200_bib8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1093\/neuonc\/noy145","article-title":"Characterization of the immune microenvironment of diffuse intrinsic pontine glioma: implications for development of immunotherapy","volume":"21","author":"Lieberman","year":"2019","journal-title":"Neuro Oncol"},{"key":"2025121006581889200_bib9","doi-asserted-by":"crossref","first-page":"2560","DOI":"10.1158\/1078-0432.CCR-18-0432","article-title":"CAR T cells targeting B7-H3, a pan-cancer antigen, demonstrate potent preclinical activity against pediatric solid tumors and brain tumors","volume":"25","author":"Majzner","year":"2019","journal-title":"Clin Cancer Res"},{"key":"2025121006581889200_bib10","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1038\/s41591-021-01404-8","article-title":"Locoregional infusion of HER2-specific CAR T cells in children and young adults with recurrent or refractory CNS tumors: an interim analysis","volume":"27","author":"Vitanza","year":"2021","journal-title":"Nat Med"},{"key":"2025121006581889200_bib11","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.ccell.2019.01.002","article-title":"Antitumor responses in the absence of toxicity in solid tumors by targeting B7-H3 via chimeric antigen receptor T cells","volume":"35","author":"Du","year":"2019","journal-title":"Cancer Cell"},{"key":"2025121006581889200_bib12","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ebiom.2019.08.030","article-title":"B7-H3-redirected chimeric antigen receptor T cells target glioblastoma and neurospheres","volume":"47","author":"Nehama","year":"2019","journal-title":"EBioMedicine"},{"key":"2025121006581889200_bib13","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.omto.2019.07.002","article-title":"B7-H3 as a novel CAR-T therapeutic target for glioblastoma","volume":"14","author":"Tang","year":"2019","journal-title":"Mol Ther Oncolytics"},{"key":"2025121006581889200_bib14","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.omto.2020.03.019","article-title":"B7-H3-targeted CAR-T cells exhibit potent antitumor effects on hematologic and solid tumors","volume":"17","author":"Zhang","year":"2020","journal-title":"Mol Ther Oncolytics"},{"key":"2025121006581889200_bib15","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1093\/neuonc\/noaa278","article-title":"Cell-surface antigen profiling of pediatric brain tumors: B7-H3 is consistently expressed and can be targeted via local or systemic CAR T-cell delivery","volume":"23","author":"Haydar","year":"2021","journal-title":"Neuro Oncol"},{"key":"2025121006581889200_bib16","doi-asserted-by":"crossref","first-page":"691741","DOI":"10.3389\/fimmu.2021.691741","article-title":"A novel orthotopic implantation technique for osteosarcoma produces spontaneous metastases and illustrates dose-dependent efficacy of B7-H3-CAR T cells","volume":"12","author":"Talbot","year":"2021","journal-title":"Front Immunol"},{"key":"2025121006581889200_bib17","doi-asserted-by":"crossref","first-page":"3834","DOI":"10.1158\/1078-0432.CCR-12-0715","article-title":"Development of an Fc-enhanced anti-B7-H3 monoclonal antibody with potent antitumor activity","volume":"18","author":"Loo","year":"2012","journal-title":"Clin Cancer Res"},{"key":"2025121006581889200_bib18","first-page":"158","article-title":"A protocol for the generation of treatment-naive biopsy-derived diffuse intrinsic pontine glioma and diffuse midline glioma models","volume":"1","author":"Biery","year":"2020","journal-title":"J Exp Neurol"},{"key":"2025121006581889200_bib19","doi-asserted-by":"crossref","first-page":"7080","DOI":"10.18632\/oncotarget.27389","article-title":"EGFR806-CAR T cells selectively target a tumor-restricted EGFR epitope in glioblastoma","volume":"10","author":"Ravanpay","year":"2019","journal-title":"Oncotarget"},{"key":"2025121006581889200_bib20","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1093\/neuonc\/noaa249","article-title":"Optimal therapeutic targeting by HDAC inhibition in biopsy-derived treatment-naive diffuse midline glioma models","volume":"23","author":"Vitanza","year":"2021","journal-title":"Neuro Oncol"},{"key":"2025121006581889200_bib21","doi-asserted-by":"crossref","first-page":"2561","DOI":"10.1056\/NEJMoa1610497","article-title":"Regression of glioblastoma after chimeric antigen receptor T-cell therapy","volume":"375","author":"Brown","year":"2016","journal-title":"N Engl J Med"},{"key":"2025121006581889200_bib22","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/s41392-021-00505-7","article-title":"Administration of B7-H3 targeted chimeric antigen receptor-T cells induce regression of glioblastoma","volume":"6","author":"Tang","year":"2021","journal-title":"Signal Transduct Target Ther"},{"key":"2025121006581889200_bib23","first-page":"4142","article-title":"Active site-directed double mutants of dihydrofolate reductase","volume":"56","author":"Ercikan-Abali","year":"1996","journal-title":"Cancer Res"},{"key":"2025121006581889200_bib24","doi-asserted-by":"crossref","first-page":"e65519","DOI":"10.1371\/journal.pone.0065519","article-title":"Engineering human T cells for resistance to methotrexate and mycophenolate mofetil as an in vivo cell selection strategy","volume":"8","author":"Jonnalagadda","year":"2013","journal-title":"PLoS One"},{"key":"2025121006581889200_bib25","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/gt.2012.97","article-title":"Efficient selection of genetically modified human T cells using methotrexate-resistant human dihydrofolate reductase","volume":"20","author":"Jonnalagadda","year":"2013","journal-title":"Gene Ther"},{"key":"2025121006581889200_bib26","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1038\/s41591-021-01564-7","article-title":"Neurocognitive and hypokinetic movement disorder with features of parkinsonism after BCMA-targeting CAR-T cell therapy","volume":"27","author":"Van Oekelen","year":"2021","journal-title":"Nat Med"},{"key":"2025121006581889200_bib27","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1126\/science.abb9847","article-title":"Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer","volume":"370","author":"Krishna","year":"2020","journal-title":"Science"},{"key":"2025121006581889200_bib28","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/j.jprot.2009.03.007","article-title":"Isotope dilution strategies for absolute quantitative proteomics","volume":"72","author":"Brun","year":"2009","journal-title":"J Proteomics"},{"key":"2025121006581889200_bib29","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1093\/clinchem\/hvz022","article-title":"Protein biomarker quantification by immunoaffinity liquid chromatography-tandem mass spectrometry: current state and future vision","volume":"66","author":"Neubert","year":"2020","journal-title":"Clin Chem"},{"key":"2025121006581889200_bib30","doi-asserted-by":"crossref","first-page":"E1074","DOI":"10.1210\/jc.2015-1967","article-title":"Thyroglobulin (Tg) testing revisited: Tg assays, TgAb assays, and correlation of results with clinical outcomes","volume":"100","author":"Netzel","year":"2015","journal-title":"J Clin Endocrinol Metab"},{"key":"2025121006581889200_bib31","doi-asserted-by":"crossref","first-page":"464","DOI":"10.3389\/fimmu.2020.00464","article-title":"Multiplexed proteomic analysis for diagnosis and screening of five primary immunodeficiency disorders from dried blood spots","volume":"11","author":"Collins","year":"2020","journal-title":"Front Immunol"},{"key":"2025121006581889200_bib32","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1093\/clinchem\/hvab047","article-title":"Quantification of human epidermal growth factor receptor 2 by immunopeptide enrichment and targeted mass spectrometry in formalin-fixed paraffin-embedded and frozen breast cancer tissues","volume":"67","author":"Kennedy","year":"2021","journal-title":"Clin Chem"},{"key":"2025121006581889200_bib33","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1373\/clinchem.2012.195594","article-title":"Measurement of thyroglobulin by liquid chromatography-tandem mass spectrometry in serum and plasma in the presence of antithyroglobulin autoantibodies","volume":"59","author":"Kushnir","year":"2013","journal-title":"Clin Chem"},{"key":"2025121006581889200_bib34","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1093\/clinchem\/hvaa178","article-title":"Clinical application of multiple reaction monitoring-mass spectrometry to human epidermal growth factor receptor 2 measurements as a potential diagnostic tool for breast cancer therapy","volume":"66","author":"Do","year":"2020","journal-title":"Clin Chem"},{"key":"2025121006581889200_bib35","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.jmoldx.2013.03.002","article-title":"Application of selected reaction monitoring for multiplex quantification of clinically validated biomarkers in formalin-fixed, paraffin-embedded tumor tissue","volume":"15","author":"Hembrough","year":"2013","journal-title":"J Mol Diagn"},{"key":"2025121006581889200_bib36","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.dnarep.2018.03.003","article-title":"Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage","volume":"65","author":"Whiteaker","year":"2018","journal-title":"DNA Repair (Amst)"},{"key":"2025121006581889200_bib37","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1667\/RR14963.1","article-title":"A multiplexed mass spectrometry-based assay for robust quantification of phosphosignaling in response to DNA damage","volume":"189","author":"Whiteaker","year":"2018","journal-title":"Radiat Res"},{"key":"2025121006581889200_bib38","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1074\/mcp.M114.047050","article-title":"Large-scale interlaboratory study to develop, analytically validate and apply highly multiplexed, quantitative peptide assays to measure cancer-relevant proteins in plasma","volume":"14","author":"Abbatiello","year":"2015","journal-title":"Mol Cell Proteomics"},{"key":"2025121006581889200_bib39","doi-asserted-by":"crossref","first-page":"2756","DOI":"10.3389\/fimmu.2018.02756","article-title":"Rapid multiplexed proteomic screening for primary immunodeficiency disorders from dried blood spots","volume":"9","author":"Collins","year":"2018","journal-title":"Front Immunol"},{"key":"2025121006581889200_bib40","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1021\/acs.jproteome.6b00245","article-title":"Optimized protocol for quantitative multiple reaction monitoring-based proteomic analysis of formalin-fixed, paraffin-embedded tissues","volume":"15","author":"Kennedy","year":"2016","journal-title":"J Proteome Res"},{"key":"2025121006581889200_bib41","doi-asserted-by":"crossref","first-page":"M111.013854","DOI":"10.1074\/mcp.M111.013854","article-title":"Interlaboratory evaluation of automated, multiplexed peptide immunoaffinity enrichment coupled to multiple reaction monitoring mass spectrometry for quantifying proteins in plasma","volume":"11","author":"Kuhn","year":"2012","journal-title":"Mol Cell Proteomics"},{"key":"2025121006581889200_bib42","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nmeth.3002","article-title":"CPTAC assay portal: a repository of targeted proteomic assays","volume":"11","author":"Whiteaker","year":"2014","journal-title":"Nat Methods"},{"key":"2025121006581889200_bib43","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/nbt.1900","article-title":"A targeted proteomics-based pipeline for verification of biomarkers in plasma","volume":"29","author":"Whiteaker","year":"2011","journal-title":"Nat Biotechnol"},{"key":"2025121006581889200_bib44","doi-asserted-by":"crossref","first-page":"M111.015347","DOI":"10.1074\/mcp.M111.015347","article-title":"Sequential multiplexed analyte quantification using peptide immunoaffinity enrichment coupled to mass spectrometry","volume":"11","author":"Whiteaker","year":"2012","journal-title":"Mol Cell Proteomics"},{"key":"2025121006581889200_bib45","first-page":"2812","article-title":"Quantification of proteins using peptide immunoaffinity enrichment coupled with mass spectrometry","volume":"53","author":"Zhao","year":"2011","journal-title":"J Vis Exp"},{"key":"2025121006581889200_bib46","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ab.2006.12.023","article-title":"Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers","volume":"362","author":"Whiteaker","year":"2007","journal-title":"Anal Biochem"},{"key":"2025121006581889200_bib47","doi-asserted-by":"crossref","first-page":"765898","DOI":"10.3389\/fimmu.2021.765898","article-title":"Targeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens","volume":"12","author":"Whiteaker","year":"2021","journal-title":"Front Immunol"},{"key":"2025121006581889200_bib48","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1158\/2326-6066.CIR-21-0501","article-title":"Modified manufacturing process modulates CD19CAR T-cell engraftment fitness and leukemia-free survival in pediatric and young adult subjects","volume":"10","author":"Ceppi","year":"2022","journal-title":"Cancer Immunol Res"},{"key":"2025121006581889200_bib49","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1002\/path.2104","article-title":"Immunosenescence of ageing","volume":"211","author":"Gruver","year":"2007","journal-title":"J Pathol"},{"key":"2025121006581889200_bib50","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.coi.2009.07.001","article-title":"Age-associated declines in immune system development and function: causes, consequences, and reversal","volume":"21","author":"Dorshkind","year":"2009","journal-title":"Curr Opin Immunol"},{"key":"2025121006581889200_bib51","doi-asserted-by":"crossref","first-page":"e1137","DOI":"10.1002\/cti2.1137","article-title":"Bioactivity and safety of B7-H3-targeted chimeric antigen receptor T cells against anaplastic meningioma","volume":"9","author":"Tang","year":"2020","journal-title":"Clin Transl Immunology"},{"key":"2025121006581889200_bib52","doi-asserted-by":"crossref","first-page":"e96976","DOI":"10.1172\/jci.insight.96976","article-title":"Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation","volume":"3","author":"Guedan","year":"2018","journal-title":"JCI Insight"},{"key":"2025121006581889200_bib53","doi-asserted-by":"crossref","first-page":"4016","DOI":"10.1038\/s41467-019-11869-4","article-title":"CXCR1- or CXCR2-modified CAR T cells co-opt IL-8 for maximal antitumor efficacy in solid tumors","volume":"10","author":"Jin","year":"2019","journal-title":"Nat Commun"},{"key":"2025121006581889200_bib54","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.1182\/blood.2020007445","article-title":"Tumor interferon signaling and suppressive myeloid cells are associated with CAR T-cell failure in large B-cell lymphoma","volume":"137","author":"Jain","year":"2021","journal-title":"Blood"},{"key":"2025121006581889200_bib55","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1038\/s41591-020-0821-8","article-title":"Locoregionally administered B7-H3-targeted CAR T cells for treatment of atypical teratoid\/rhabdoid tumors","volume":"26","author":"Theruvath","year":"2020","journal-title":"Nat Med"},{"key":"2025121006581889200_bib56","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1038\/s41591-020-0827-2","article-title":"Locoregional delivery of CAR T cells to the cerebrospinal fluid for treatment of metastatic medulloblastoma and ependymoma","volume":"26","author":"Donovan","year":"2020","journal-title":"Nat Med"},{"key":"2025121006581889200_bib57","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1056\/NEJMoa2202028","article-title":"Oncolytic DNX-2401 virus for pediatric diffuse intrinsic pontine glioma","volume":"386","author":"Gallego Perez-Larraya","year":"2022","journal-title":"N Engl J Med"},{"key":"2025121006581889200_bib58","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1158\/1078-0432.CCR-13-0330","article-title":"Receptor affinity and extracellular domain modifications affect tumor recognition by ROR1-specific chimeric antigen receptor T cells","volume":"19","author":"Hudecek","year":"2013","journal-title":"Clin Cancer Res"},{"key":"2025121006581889200_bib59","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1158\/2326-6066.CIR-14-0200","article-title":"Functional tuning of CARs reveals signaling threshold above which CD8+ CTL antitumor potency is attenuated due to cell Fas-FasL-dependent AICD","volume":"3","author":"Kunkele","year":"2015","journal-title":"Cancer Immunol Res"},{"key":"2025121006581889200_bib60","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1182\/blood-2011-02-337360","article-title":"A transgene-encoded cell surface polypeptide for selection, in vivo tracking, and ablation of engineered cells","volume":"118","author":"Wang","year":"2011","journal-title":"Blood"},{"key":"2025121006581889200_bib61","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.omtm.2018.12.009","article-title":"Engineering and design of chimeric antigen receptors","volume":"12","author":"Guedan","year":"2019","journal-title":"Mol Ther Methods Clin Dev"},{"key":"2025121006581889200_bib62","doi-asserted-by":"crossref","first-page":"e1253656","DOI":"10.1080\/2162402X.2016.1253656","article-title":"Fine-tuning the CAR spacer improves T-cell potency","volume":"5","author":"Watanabe","year":"2016","journal-title":"Oncoimmunology"},{"key":"2025121006581889200_bib63","first-page":"e3683","article-title":"Determining optimal cytotoxic activity of human Her2neu specific CD8 T cells by comparing the Cr51 release assay to the xCELLigence system","volume":"66","author":"Erskine","year":"2012","journal-title":"J Vis Exp"},{"key":"2025121006581889200_bib64","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.immuni.2018.01.011","article-title":"High-dimensional single-cell mapping of central nervous system immune cells reveals distinct myeloid subsets in health, aging, and disease","volume":"48","author":"Mrdjen","year":"2018","journal-title":"Immunity"},{"key":"2025121006581889200_bib65","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/s41592-019-0654-x","article-title":"Orchestrating single-cell analysis with Bioconductor","volume":"17","author":"Amezquita","year":"2020","journal-title":"Nat Methods"},{"key":"2025121006581889200_bib66","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant graphics for data analysis","author":"Wickham","year":"2016"},{"key":"2025121006581889200_bib67","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1093\/bioinformatics\/btq054","article-title":"Skyline: an open source document editor for creating and analyzing targeted proteomics experiments","volume":"26","author":"MacLean","year":"2010","journal-title":"Bioinformatics"},{"key":"2025121006581889200_bib68","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/mas.21540","article-title":"The Skyline ecosystem: informatics for quantitative mass spectrometry proteomics","volume":"39","author":"Pino","year":"2020","journal-title":"Mass Spectrom Rev"}],"container-title":["Cancer Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/doi\/10.1158\/2159-8290.CD-22-0750\/3213955\/cd-22-0750.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/doi\/10.1158\/2159-8290.CD-22-0750\/3232584\/cd-22-0750.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/13\/1\/114\/3238350\/114.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/13\/1\/114\/3238350\/114.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T11:58:42Z","timestamp":1765367922000},"score":1,"resource":{"primary":{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article\/13\/1\/114\/712703\/Intraventricular-B7-H3-CAR-T-Cells-for-Diffuse"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,19]]},"references-count":68,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,10,19]]},"published-print":{"date-parts":[[2023,1,9]]}},"URL":"https:\/\/doi.org\/10.1158\/2159-8290.cd-22-0750","relation":{"has-preprint":[{"id-type":"doi","id":"10.1158\/2159-8290.22541686.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.c.6549724","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.c.6549724.v1","asserted-by":"object"}],"is-supplemented-by":[{"id-type":"doi","id":"10.1158\/2159-8290.22541686","asserted-by":"object"}]},"ISSN":["2159-8274","2159-8290"],"issn-type":[{"value":"2159-8274","type":"print"},{"value":"2159-8290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,19]]}}}