{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T21:35:46Z","timestamp":1793223346316,"version":"4.5.0"},"reference-count":58,"publisher":"American Association for Cancer Research (AACR)","issue":"9","funder":[{"name":"National Cancer Institute","award":["R35CA242376"],"award-info":[{"award-number":["R35CA242376"]}]},{"name":"Leukemia and Lymphoma Society","award":["7020-19"],"award-info":[{"award-number":["7020-19"]}],"id":[{"id":"https:\/\/ror.org\/04m0xav37","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"crossref","award":["BX004768-01"],"award-info":[{"award-number":["BX004768-01"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["aacrjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,9,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>The BCL2 inhibitor venetoclax has recently emerged as an important component of acute myeloid leukemia (AML) therapy. Notably, use of this agent has revealed a previously unrecognized form of pathogenesis characterized by monocytic disease progression. We demonstrate that this form of disease arises from a fundamentally different type of leukemia stem cell (LSC), which we designate as monocytic LSC (m-LSC), that is developmentally and clinically distinct from the more well-described primitive LSC (p-LSC). The m-LSC is distinguished by a unique immunophenotype (CD34\u2212, CD4+, CD11b\u2212, CD14\u2212, CD36\u2212), unique transcriptional state, reliance on purine metabolism, and selective sensitivity to cladribine. Critically, in some instances, m-LSC and p-LSC subtypes can co-reside in the same patient with AML and simultaneously contribute to overall tumor biology. Thus, our findings demonstrate that LSC heterogeneity has direct clinical significance and highlight the need to distinguish and target m-LSCs as a means to improve clinical outcomes with venetoclax-based regimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance:<\/jats:title>\n                    <jats:p>These studies identify and characterize a new type of human acute myeloid LSC that is responsible for monocytic disease progression in patients with AML treated with venetoclax-based regimens. Our studies describe the phenotype, molecular properties, and drug sensitivities of this unique LSC subclass.<\/jats:p>\n                    <jats:p>This article is featured in Selected Articles from This Issue, p. 1949<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1158\/2159-8290.cd-22-1297","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T08:46:47Z","timestamp":1687769207000},"page":"2032-2049","update-policy":"https:\/\/doi.org\/10.1158\/crossmark_policy","source":"Crossref","is-referenced-by-count":117,"title":["A Novel Type of Monocytic Leukemia Stem Cell Revealed by the Clinical Use of Venetoclax-Based Therapy"],"prefix":"10.1158","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9619-2614","authenticated-orcid":false,"given":"Shanshan","family":"Pei","sequence":"first","affiliation":[{"name":"1Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China."},{"name":"2Liangzhu Laboratory, Zhejiang University, Hangzhou, China."},{"name":"3Institute of Hematology, Zhejiang University, Hangzhou, China."},{"name":"4Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5974-2736","authenticated-orcid":false,"given":"Ian T.","family":"Shelton","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2928-6308","authenticated-orcid":false,"given":"Austin E.","family":"Gillen","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."},{"name":"6Rocky Mountain Regional VA Medical Center, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-903X","authenticated-orcid":false,"given":"Brett M.","family":"Stevens","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2129-7253","authenticated-orcid":false,"given":"Maura","family":"Gasparetto","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4947-8665","authenticated-orcid":false,"given":"Yanan","family":"Wang","sequence":"additional","affiliation":[{"name":"1Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China."},{"name":"2Liangzhu Laboratory, Zhejiang University, Hangzhou, China."},{"name":"3Institute of Hematology, Zhejiang University, Hangzhou, China."},{"name":"4Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5537-6353","authenticated-orcid":false,"given":"Lina","family":"Liu","sequence":"additional","affiliation":[{"name":"1Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China."},{"name":"2Liangzhu Laboratory, Zhejiang University, Hangzhou, China."},{"name":"3Institute of Hematology, Zhejiang University, Hangzhou, China."},{"name":"4Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4889-2269","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"1Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China."},{"name":"2Liangzhu Laboratory, Zhejiang University, Hangzhou, China."},{"name":"3Institute of Hematology, Zhejiang University, Hangzhou, China."},{"name":"4Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2228-2198","authenticated-orcid":false,"given":"Tonya M.","family":"Brunetti","sequence":"additional","affiliation":[{"name":"7University of Colorado Cancer Center, University of Colorado Anschutz Medical Campus, Aurora, Colorado."},{"name":"8Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3132-1491","authenticated-orcid":false,"given":"Krysta","family":"Engel","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8283-055X","authenticated-orcid":false,"given":"Sarah","family":"Staggs","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5989-8570","authenticated-orcid":false,"given":"William","family":"Showers","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8201-2387","authenticated-orcid":false,"given":"Anagha Inguva","family":"Sheth","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9503-5019","authenticated-orcid":false,"given":"Maria L.","family":"Amaya","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."},{"name":"6Rocky Mountain Regional VA Medical Center, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9179-1540","authenticated-orcid":false,"given":"Mohammad","family":"Minhajuddin","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7916-7505","authenticated-orcid":false,"given":"Amanda","family":"Winters","sequence":"additional","affiliation":[{"name":"9Center for Cancer and Blood Disorders, Department of Pediatrics, University of Colorado, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7082-7204","authenticated-orcid":false,"given":"Sweta B.","family":"Patel","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8743-7840","authenticated-orcid":false,"given":"Hunter","family":"Tolison","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2961-3999","authenticated-orcid":false,"given":"Anna E.","family":"Krug","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6635-3303","authenticated-orcid":false,"given":"Tracy N.","family":"Young","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8653-3820","authenticated-orcid":false,"given":"Jeffrey","family":"Schowinsky","sequence":"additional","affiliation":[{"name":"10Department of Pathology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9745-9595","authenticated-orcid":false,"given":"Christine M.","family":"McMahon","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6478-4244","authenticated-orcid":false,"given":"Clayton A.","family":"Smith","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6519-4860","authenticated-orcid":false,"given":"Daniel A.","family":"Pollyea","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0869-3465","authenticated-orcid":false,"given":"Craig T.","family":"Jordan","sequence":"additional","affiliation":[{"name":"5Division of Hematology, University of Colorado School of Medicine, Aurora, Colorado."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1086","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"key":"2025121107015256700_bib1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.stem.2014.02.006","article-title":"Evolution of the cancer stem cell model","volume":"14","author":"Kreso","year":"2014","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib2","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1182\/blood-2016-10-696039","article-title":"Therapeutic targeting of acute myeloid leukemia stem cells","volume":"129","author":"Pollyea","year":"2017","journal-title":"Blood"},{"key":"2025121107015256700_bib3","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1182\/blood-2016-10-696054","article-title":"Biology and relevance of human acute myeloid leukemia stem cells","volume":"129","author":"Thomas","year":"2017","journal-title":"Blood"},{"key":"2025121107015256700_bib4","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/nm0797-730","article-title":"Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell","volume":"3","author":"Bonnet","year":"1997","journal-title":"Nat Med"},{"key":"2025121107015256700_bib5","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/ni1080","article-title":"Acute myeloid leukemia originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity","volume":"5","author":"Hope","year":"2004","journal-title":"Nat Immunol"},{"key":"2025121107015256700_bib6","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1172\/JCI41495","article-title":"Human acute myelogenous leukemia stem cells are rare and heterogeneous when assayed in NOD\/SCID\/IL2Rgammac-deficient mice","volume":"121","author":"Sarry","year":"2011","journal-title":"J Clin Invest"},{"key":"2025121107015256700_bib7","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1182\/blood-2016-02-695312","article-title":"Evolution of acute myelogenous leukemia stem cell properties after treatment and progression","volume":"128","author":"Ho","year":"2016","journal-title":"Blood"},{"key":"2025121107015256700_bib8","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1084\/jem.20151775","article-title":"Genetically distinct leukemic stem cells in human CD34-acute myeloid leukemia are arrested at a hemopoietic precursor-like stage","volume":"213","author":"Quek","year":"2016","journal-title":"J Exp Med"},{"key":"2025121107015256700_bib9","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ccr.2010.12.012","article-title":"Coexistence of LMPP-like and GMP-like leukemia stem cells in acute myeloid leukemia","volume":"19","author":"Goardon","year":"2011","journal-title":"Cancer Cell"},{"key":"2025121107015256700_bib10","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/s41586-020-2864-x","article-title":"Single-cell mutation analysis of clonal evolution in myeloid malignancies","volume":"587","author":"Miles","year":"2020","journal-title":"Nature"},{"key":"2025121107015256700_bib11","doi-asserted-by":"crossref","first-page":"5327","DOI":"10.1038\/s41467-020-19119-8","article-title":"Clonal evolution of acute myeloid leukemia revealed by high-throughput single-cell genomics","volume":"11","author":"Morita","year":"2020","journal-title":"Nat Commun"},{"key":"2025121107015256700_bib12","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1158\/2159-8290.CD-19-0710","article-title":"Monocytic subclones confer resistance to venetoclax-based therapy in patients with acute myeloid leukemia","volume":"10","author":"Pei","year":"2020","journal-title":"Cancer Discov"},{"key":"2025121107015256700_bib13","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2141.1976.tb03563.x","article-title":"Proposals for the classification of the acute leukaemias. French-American-British (FAB) co-operative group","volume":"33","author":"Bennett","year":"1976","journal-title":"Br J Haematol"},{"key":"2025121107015256700_bib14","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1038\/s41591-022-01819-x","article-title":"A cellular hierarchy framework for understanding heterogeneity and predicting drug response in acute myeloid leukemia","volume":"28","author":"Zeng","year":"2022","journal-title":"Nat Med"},{"key":"2025121107015256700_bib15","doi-asserted-by":"crossref","first-page":"223","DOI":"10.12688\/f1000research.22969.2","article-title":"clustifyr: an R package for automated single-cell RNA sequencing cluster classification","volume":"9","author":"Fu","year":"2020","journal-title":"F1000Res."},{"key":"2025121107015256700_bib16","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.cell.2011.01.004","article-title":"Densely interconnected transcriptional circuits control cell states in human hematopoiesis","volume":"144","author":"Novershtern","year":"2011","journal-title":"Cell"},{"key":"2025121107015256700_bib17","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/s41587-021-01001-7","article-title":"Mapping single-cell data to reference atlases by transfer learning","volume":"40","author":"Lotfollahi","year":"2022","journal-title":"Nat Biotechnol"},{"key":"2025121107015256700_bib18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.ccr.2006.08.020","article-title":"Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia","volume":"10","author":"Somervaille","year":"2006","journal-title":"Cancer Cell"},{"key":"2025121107015256700_bib19","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.stem.2008.11.015","article-title":"Hierarchical maintenance of MLL myeloid leukemia stem cells employs a transcriptional program shared with embryonic rather than adult stem cells","volume":"4","author":"Somervaille","year":"2009","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib20","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1182\/blood-2005-12-5014","article-title":"c-Myb is an essential downstream target for homeobox-mediated transformation of hematopoietic cells","volume":"108","author":"Hess","year":"2006","journal-title":"Blood"},{"key":"2025121107015256700_bib21","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/nature04980","article-title":"Transformation from committed progenitor to leukaemia stem cell initiated by MLL-AF9","volume":"442","author":"Krivtsov","year":"2006","journal-title":"Nature"},{"key":"2025121107015256700_bib22","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1182\/blood-2003-02-0434","article-title":"AML with 11q23\/MLL abnormalities as defined by the WHO classification: incidence, partner chromosomes, FAB subtype, age distribution, and prognostic impact in an unselected series of 1897 cytogenetically analyzed AML cases","volume":"102","author":"Schoch","year":"2003","journal-title":"Blood"},{"key":"2025121107015256700_bib23","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.stem.2015.08.008","article-title":"CD93 marks a non-quiescent human leukemia stem cell population and is required for development of MLL-rearranged acute myeloid leukemia","volume":"17","author":"Iwasaki","year":"2015","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib24","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/nature20598","article-title":"A 17-gene stemness score for rapid determination of risk in acute leukaemia","volume":"540","author":"Ng","year":"2016","journal-title":"Nature"},{"key":"2025121107015256700_bib25","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1038\/nm.2415","article-title":"Stem cell gene expression programs influence clinical outcome in human leukemia","volume":"17","author":"Eppert","year":"2011","journal-title":"Nat Med"},{"key":"2025121107015256700_bib26","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.3390\/ijms21103483","article-title":"Overview of dual-acting drug methotrexate in different neurological diseases, autoimmune pathologies and cancers","volume":"21","author":"Kozminski","year":"2020","journal-title":"Int J Mol Sci"},{"key":"2025121107015256700_bib27","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.cell.2016.08.057","article-title":"Inhibition of dihydroorotate dehydrogenase overcomes differentiation blockade in acute myeloid leukemia","volume":"167","author":"Sykes","year":"2016","journal-title":"Cell"},{"key":"2025121107015256700_bib28","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1182\/blood-2010-02-246140","article-title":"Beyond hairy cell: the activity of cladribine in other hematologic malignancies","volume":"116","author":"Sigal","year":"2010","journal-title":"Blood"},{"key":"2025121107015256700_bib29","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1182\/blood-2015-11-683649","article-title":"GPR56 identifies primary human acute myeloid leukemia cells with high repopulating potential in vivo","volume":"127","author":"Pabst","year":"2016","journal-title":"Blood"},{"key":"2025121107015256700_bib30","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1158\/2159-8290.CD-22-0939","article-title":"Combinatorial BCL2 family expression in acute myeloid leukemia stem cells predicts clinical response to azacitidine\/venetoclax","volume":"13","author":"Waclawiczek","year":"2023","journal-title":"Cancer Discov"},{"key":"2025121107015256700_bib31","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1038\/s41591-018-0233-1","article-title":"Venetoclax with azacitidine disrupts energy metabolism and targets leukemia stem cells in patients with acute myeloid leukemia","volume":"24","author":"Pollyea","year":"2018","journal-title":"Nat Med"},{"key":"2025121107015256700_bib32","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.stem.2020.07.021","article-title":"Nicotinamide metabolism mediates resistance to venetoclax in relapsed acute myeloid leukemia stem cells","volume":"27","author":"Jones","year":"2020","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib33","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.ccell.2018.10.005","article-title":"Inhibition of amino acid metabolism selectively targets human leukemia stem cells","volume":"34","author":"Jones","year":"2018","journal-title":"Cancer Cell"},{"key":"2025121107015256700_bib34","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.stem.2021.02.013","article-title":"Targeting energy metabolism in cancer stem cells: progress and challenges in leukemia and solid tumors","volume":"28","author":"Jones","year":"2021","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib35","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1038\/s43018-020-00126-z","article-title":"Fatty acid metabolism underlies venetoclax resistance in acute myeloid leukemia stem cells","volume":"1","author":"Stevens","year":"2020","journal-title":"Nat Cancer"},{"key":"2025121107015256700_bib36","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.stem.2016.06.001","article-title":"Leukemic stem cells evade chemotherapy by metabolic adaptation to an adipose tissue niche","volume":"19","author":"Ye","year":"2016","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib37","doi-asserted-by":"crossref","first-page":"2768","DOI":"10.1200\/JCO.20.03736","article-title":"Venetoclax combined with FLAG-IDA induction and consolidation in newly diagnosed and relapsed or refractory acute myeloid leukemia","volume":"39","author":"DiNardo","year":"2021","journal-title":"J Clin Oncol"},{"key":"2025121107015256700_bib38","doi-asserted-by":"crossref","first-page":"e552","DOI":"10.1016\/S2352-3026(21)00192-7","article-title":"Venetoclax plus intensive chemotherapy with cladribine, idarubicin, and cytarabine in patients with newly diagnosed acute myeloid leukaemia or high-risk myelodysplastic syndrome: a cohort from a single-centre, single-arm, phase 2 trial","volume":"8","author":"Kadia","year":"2021","journal-title":"Lancet Haematol"},{"key":"2025121107015256700_bib39","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1200\/JCO.21.02823","article-title":"Phase II study of venetoclax added to cladribine plus low-dose cytarabine alternating with 5-azacitidine in older patients with newly diagnosed acute myeloid leukemia","volume":"40","author":"Kadia","year":"2022","journal-title":"J Clin Oncol"},{"key":"2025121107015256700_bib40","doi-asserted-by":"crossref","first-page":"e415","DOI":"10.1016\/S2352-3026(22)00106-5","article-title":"Venetoclax plus 3 + 7 daunorubicin and cytarabine chemotherapy as first-line treatment for adults with acute myeloid leukaemia: a multicentre, single-arm, phase 2 trial","volume":"9","author":"Wang","year":"2022","journal-title":"Lancet Haematol"},{"key":"2025121107015256700_bib41","doi-asserted-by":"crossref","first-page":"3245","DOI":"10.1080\/10428194.2022.2113530","article-title":"Dismal survival outcomes of patients with acute myeloid leukemia after failure of venetoclax with hypomethylating agents","volume":"63","author":"Zainaldin","year":"2022","journal-title":"Leuk Lymphoma"},{"key":"2025121107015256700_bib42","doi-asserted-by":"crossref","first-page":"894","DOI":"10.3324\/haematol.2020.252569","article-title":"Outcomes of relapsed or refractory acute myeloid leukemia after frontline hypomethylating agent and venetoclax regimens","volume":"106","author":"Maiti","year":"2021","journal-title":"Haematologica"},{"key":"2025121107015256700_bib43","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1038\/s43018-020-0103-x","article-title":"Integrated analysis of patient samples identifies biomarkers for venetoclax efficacy and combination strategies in acute myeloid leukemia","volume":"1","author":"Zhang","year":"2020","journal-title":"Nat Cancer"},{"key":"2025121107015256700_bib44","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1101\/gad.1143403","article-title":"Similar MLL-associated leukemias arising from self-renewing stem cells and short-lived myeloid progenitors","volume":"17","author":"Cozzio","year":"2003","journal-title":"Genes Dev"},{"key":"2025121107015256700_bib45","doi-asserted-by":"crossref","first-page":"eabc4822","DOI":"10.1126\/scitranslmed.abc4822","article-title":"Functional reconstruction of human AML reveals stem cell origin and vulnerability of treatment-resistant MLL-rearranged leukemia","volume":"13","author":"Zeisig","year":"2021","journal-title":"Sci Transl Med"},{"key":"2025121107015256700_bib46","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1182\/blood.2019003988","article-title":"Molecular patterns of response and treatment failure after frontline venetoclax combinations in older patients with AML","volume":"135","author":"DiNardo","year":"2020","journal-title":"Blood"},{"key":"2025121107015256700_bib47","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/s41591-022-01696-4","article-title":"Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy","volume":"28","author":"Ganan-Gomez","year":"2022","journal-title":"Nat Med"},{"key":"2025121107015256700_bib48","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.stem.2018.05.021","article-title":"AMPK\/FIS1-mediated mitophagy is required for self-renewal of human AML stem cells","volume":"23","author":"Pei","year":"2018","journal-title":"Cell Stem Cell"},{"key":"2025121107015256700_bib49","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"2025121107015256700_bib50","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1186\/s13059-020-02084-2","article-title":"GMM-Demux: sample demultiplexing, multiplet detection, experiment planning, and novel cell-type verification in single cell sequencing","volume":"21","author":"Xin","year":"2020","journal-title":"Genome Biol"},{"key":"2025121107015256700_bib51","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1101\/gr.271908.120","article-title":"Automated quality control and cell identification of droplet-based single-cell data using dropkick","volume":"31","author":"Heiser","year":"2021","journal-title":"Genome Res"},{"key":"2025121107015256700_bib52","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s13059-020-1950-6","article-title":"Decontamination of ambient RNA in single-cell RNA-seq with DecontX","volume":"21","author":"Yang","year":"2020","journal-title":"Genome Biol"},{"key":"2025121107015256700_bib53","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: doublet dete\u00adction in single-cell RNA sequencing data using artificial nearest neighbors","volume":"8","author":"McGinnis","year":"2019","journal-title":"Cell Syst"},{"key":"2025121107015256700_bib54","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/s41592-020-01050-x","article-title":"Joint probabilistic modeling of single-cell multi-omic data with totalVI","volume":"18","author":"Gayoso","year":"2021","journal-title":"Nat Methods"},{"key":"2025121107015256700_bib55","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s13059-017-1382-0","article-title":"SCANPY: large-scale single-cell gene expression data analysis","volume":"19","author":"Wolf","year":"2018","journal-title":"Genome Biol"},{"key":"2025121107015256700_bib56","doi-asserted-by":"crossref","first-page":"5233","DOI":"10.1038\/s41598-019-41695-z","article-title":"From Louvain to Leiden: guaranteeing well-connected communities","volume":"9","author":"Traag","year":"2019","journal-title":"Sci Rep."},{"key":"2025121107015256700_bib57","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1038\/s41590-021-01059-0","article-title":"Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states","volume":"22","author":"Triana","year":"2021","journal-title":"Nat Immunol"},{"key":"2025121107015256700_bib58","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"}],"container-title":["Cancer Discovery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/doi\/10.1158\/2159-8290.CD-22-1297\/3342626\/cd-22-1297.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/doi\/10.1158\/2159-8290.CD-22-1297\/3356404\/cd-22-1297.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/13\/9\/2032\/3362261\/2032.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article-pdf\/13\/9\/2032\/3362261\/2032.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T12:02:09Z","timestamp":1765454529000},"score":1,"resource":{"primary":{"URL":"https:\/\/aacrjournals.org\/cancerdiscovery\/article\/13\/9\/2032\/728867\/A-Novel-Type-of-Monocytic-Leukemia-Stem-Cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,26]]},"references-count":58,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,6,26]]},"published-print":{"date-parts":[[2023,9,6]]}},"URL":"https:\/\/doi.org\/10.1158\/2159-8290.cd-22-1297","relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.1158\/2159-8290.27025859.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091960.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091954.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091945","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025871.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091948.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091951","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025862.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025865.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091939","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025868.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091951.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025874.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091957.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091957","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859695","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025865","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025877","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025856.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859704","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859710","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025871","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091942","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091954","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091960","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859707","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025859","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091948","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025877.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025862","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091942.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025874","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091939.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.24091945.v1","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859692","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025856","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.27025868","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859713","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859701","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.30859698","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.1158\/2159-8290.c.6822169","asserted-by":"object"},{"id-type":"doi","id":"10.1101\/2022.12.04.519036","asserted-by":"object"},{"id-type":"doi","id":"10.1158\/2159-8290.c.6822169.v1","asserted-by":"object"}]},"ISSN":["2159-8274","2159-8290"],"issn-type":[{"value":"2159-8274","type":"print"},{"value":"2159-8290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,26]]}}}