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In the absence of serum, wild-type cells showed slower growth, which was accompanied by a marked decrease in phosphocholine concentration, which was not observed in otherwise isogenic cell lines lacking p300. In the presence of serum, several metabolites were identified as being significantly different between the two cell types, including glutamate and glutamine, a nicotinamide-related compound and glycerophosphocholine (GPC). However, in the absence of serum, these metabolites, with the exception of GPC, were not significantly different, leading us to conclude that most of these changes were context dependent. Transcript profiling, using DNA microarrays, showed changes in the levels of transcripts for several enzymes involved in choline metabolism, which might explain the change in GPC concentration. Localized in vivo\u20081H NMR measurements on the tumors formed following s.c. implantation of these cells into mice showed an increase in the intensity of the peak from choline-containing compounds in the p300\u2212 tumors. These data show that NMR-based metabolite profiling has sufficient sensitivity to identify the metabolic consequences of p300 gene deletion in tumor cells in vitro and in vivo. (Cancer Res 2006; 66(15): 7606-14)<\/jats:p>","DOI":"10.1158\/0008-5472.can-05-2999","type":"journal-article","created":{"date-parts":[[2006,8,2]],"date-time":"2006-08-02T19:55:38Z","timestamp":1154548538000},"page":"7606-7614","update-policy":"https:\/\/doi.org\/10.1158\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Metabolic Consequences of p300 Gene Deletion in Human Colon Cancer Cells"],"prefix":"10.1158","volume":"66","author":[{"given":"Jacob G.","family":"Bundy","sequence":"first","affiliation":[{"name":"1Department of Biochemistry and"}]},{"given":"N. Gopalakrishna","family":"Iyer","sequence":"additional","affiliation":[{"name":"2Cancer Genomics Program, Department of Oncology, Hutchison\/Medical Research Council Research Centre, University of Cambridge, Cambridge, United Kingdom"}]},{"given":"Michelle S.","family":"Gentile","sequence":"additional","affiliation":[{"name":"2Cancer Genomics Program, Department of Oncology, Hutchison\/Medical Research Council Research Centre, University of Cambridge, Cambridge, United Kingdom"}]},{"given":"De-En","family":"Hu","sequence":"additional","affiliation":[{"name":"1Department of Biochemistry and"}]},{"given":"Mikko","family":"Kettunen","sequence":"additional","affiliation":[{"name":"1Department of Biochemistry and"}]},{"given":"Ana-Teresa","family":"Maia","sequence":"additional","affiliation":[{"name":"2Cancer Genomics Program, Department of Oncology, Hutchison\/Medical Research Council Research Centre, University of Cambridge, Cambridge, United Kingdom"}]},{"given":"Natalie P.","family":"Thorne","sequence":"additional","affiliation":[{"name":"2Cancer Genomics Program, Department of Oncology, Hutchison\/Medical Research Council Research Centre, University of Cambridge, Cambridge, United Kingdom"}]},{"given":"James D.","family":"Brenton","sequence":"additional","affiliation":[{"name":"2Cancer Genomics Program, Department of Oncology, Hutchison\/Medical Research Council Research Centre, University of Cambridge, Cambridge, United Kingdom"}]},{"given":"Carlos","family":"Caldas","sequence":"additional","affiliation":[{"name":"2Cancer Genomics Program, Department of Oncology, Hutchison\/Medical Research Council Research Centre, University of Cambridge, Cambridge, United Kingdom"}]},{"given":"Kevin M.","family":"Brindle","sequence":"additional","affiliation":[{"name":"1Department of Biochemistry and"}]}],"member":"1086","published-online":{"date-parts":[[2006,8,2]]},"reference":[{"key":"2022061622125081300_B1","doi-asserted-by":"crossref","unstructured":"Giordano A, Avantaggiati ML. p300 and CBP: partners for life and death. 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