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In this work, we report that loss of ER\u03b2 is associated with a decrease of E-cadherin protein levels through different posttranscriptional regulatory mechanisms. Ligand activation of ER\u03b1 induced E-cadherin extracellular shedding and internalization only in the absence of ER\u03b2, followed by lysosomal degradation. Loss of ER\u03b2 also led to an increase of E-cadherin uptake in a ligand-independent manner through mechanisms that required caveolae formation. Proteasome activity was necessary for both mechanisms to operate. Increased E-cadherin internalization correlated with the up-regulation of \u03b2-catenin transcriptional activity and impaired morphogenesis on Engelbreth-Holm-Swarm matrix. Taken together, these results emphasize the role of epithelial ER\u03b2 in maintaining cell adhesion and a differentiated phenotype and highlight the potential importance of ER\u03b2 for the design of specific agonists for use in breast cancer therapy. 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