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Because the latter approach is not representative of an in vivo steady state, we investigated antitumor activity of curcumin, 3,3\u2032-diindolylmethane (DIM), epigallocatechin gallate (EGCG), genistein, or indole-3-carbinol (I3C) in breast cancer MDA-MB-231 cells, exposed in long-term culture to low concentrations, achievable in vivo. Curcumin and EGCG increased cell doubling time. Curcumin, EGCG, and I3C inhibited clonogenic growth by 55% to 60% and induced 1.5- to 2-fold higher levels of the basal caspase-3\/7 activity. No changes in expression of cell cycle\u2013related proteins or survivin were found; however, I3C reduced epidermal growth factor receptor expression, contributing to apoptosis. Because some phytochemicals are shown to inhibit DNA and histone modification, modulation of expression by the agents in a set of genes (cadherin-11, p21Cip1, urokinase-type plasminogen activator, and interleukin-6) was compared with changes induced by inhibitors of DNA methylation or histone deacetylation. The phytochemicals modified protein and\/or RNA expression of these genes, with EGCG eliciting the least and DIM the most changes in gene expression. DIM and curcumin decreased cadherin-11 and increased urokinase-type plasminogen activator levels correlated with increased cell motility. Curcumin, DIM, EGCG, and genistein reduced cell sensitivity to radiation-induced DNA damage without affecting DNA repair. This model has revealed that apoptosis and not arrest is likely to be responsible for growth inhibition. It also implicated new molecular targets and activities of the agents under conditions relevant to human exposure. [Mol Cancer Ther 2007;6(11):3071\u20139]<\/jats:p>","DOI":"10.1158\/1535-7163.mct-07-0117","type":"journal-article","created":{"date-parts":[[2007,11,19]],"date-time":"2007-11-19T15:43:20Z","timestamp":1195487000000},"page":"3071-3079","update-policy":"https:\/\/doi.org\/10.1158\/crossmark_policy","source":"Crossref","is-referenced-by-count":98,"title":["Extended treatment with physiologic concentrations of dietary phytochemicals results in altered gene expression, reduced growth, and apoptosis of cancer cells"],"prefix":"10.1158","volume":"6","author":[{"given":"Elena P.","family":"Moiseeva","sequence":"first","affiliation":[{"name":"1Cancer Biomarkers and Prevention Group and"}]},{"given":"Gabriela M.","family":"Almeida","sequence":"additional","affiliation":[{"name":"2Radiation and Oxidative Stress Group, Department of Cancer Studies, University of Leicester, Leicester, United Kingdom"}]},{"given":"George D.D.","family":"Jones","sequence":"additional","affiliation":[{"name":"2Radiation and Oxidative Stress Group, Department of Cancer Studies, University of Leicester, Leicester, United Kingdom"}]},{"given":"Margaret M.","family":"Manson","sequence":"additional","affiliation":[{"name":"1Cancer Biomarkers and Prevention Group and"}]}],"member":"1086","published-online":{"date-parts":[[2007,11,19]]},"reference":[{"key":"2022060804424220400_BIB1","doi-asserted-by":"crossref","unstructured":"McCullough ML, Giovannucci EL. 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