{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:13:39Z","timestamp":1745554419054,"version":"3.40.4"},"reference-count":3,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[1998]]},"abstract":"<b>Background:<\/b> <i>Helicobacter pylori<\/i>, which causes gastritis and peptic ulcer, seems to be an important factor in the pathogenesis of gastric cancer and MALT lymphoma. Thus our aim was to examine whether <i>H.\u00a0pylori<\/i> influences DNA synthesis in epithelial cells in vitro. <b>Methods:<\/b> Sonicated and water extracts of <i>H.\u00a0pylori<\/i> (cytotoxic strains NCTC 11637, 88-23 and A5, and a noncytotoxic isogenic mutant of A5, A5 vac A) were diluted to a final concentration of 1\/1,000, 1\/100, 1\/50 and 1\/10. Water extracts of <i>Escherichia coli<\/i> were used as reference. IEC-6 cells were incubated during 24\u00a0h with fragments of <i>H.\u00a0pylori<\/i> or extracts of the concentrations described above. The cells were labeled with <sup>3<\/sup>H-methylthymidine for 4\u00a0h and processed for autoradiography. DNA synthesis was evaluated by the labeling index (LI). <b>Results:<\/b> The LI% of controls was 15.6 \u00b1 5.1%. All the water extracts and sonicated strains of <i>H.\u00a0pylori<\/i> increased the LI% in a dose-dependent manner (p < 0.001). The highest concentrations of the sonicated strains tended to reduce the LI%, although these values were still higher than those of the control group. The water extracts of <i>E.\u00a0coli<\/i> increased the LI% in a dose-dependent manner (p < 0.0001). <b>Conclusion:<\/b> <i>H.\u00a0pylori<\/i> stimulates DNA synthesis in epithelial cells in vitro, but no association was found with the presence of cytotoxin production. Our results suggest that hitherto unknown components of <i>H.\u00a0pylori<\/i> may contribute to the increase in cell proliferation observed in gastritis and to the development of MALT lymphoma and gastric cancer.<\/jats:p>","DOI":"10.1159\/000007464","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:42:49Z","timestamp":1050421369000},"page":"33-39","source":"Crossref","is-referenced-by-count":10,"title":["Helicobacter pylori Stimulates DNA Synthesis in a Small Intestinal Cell Line in vitro"],"prefix":"10.1159","volume":"59","author":[{"given":"Johanna","family":"Br\u00e4nnstr\u00f6m","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Zachrisson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Hult\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Engstrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s","family":"Uribe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,1,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301803"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92035-Z"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90938-D"}],"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/7464","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/7464","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:39:10Z","timestamp":1745469550000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000007464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":3,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1998,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000007464","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[1998]]}}}