{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:13:42Z","timestamp":1745554422406,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"2-3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Digestion"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; &lt;sup&gt;13&lt;\/sup&gt;C-hiolein is a purified algal triglyceride which is synthesized with 98% of all carbon atoms of the molecule uniformly &lt;sup&gt;13&lt;\/sup&gt;C-labeled. Orlistat is the new name for tetrahydrolipstatin (THL), which is a specific and potent inhibitor of gastrointestinal lipases which induces artificial fat malabsorption in humans. The aim of the present study was to use the &lt;sup&gt;13&lt;\/sup&gt;C-hiolein breath test (HBT) to assess THL-induced fat malabsorption in healthy volunteers. &lt;i&gt;Methods:&lt;\/i&gt; Eight healthy volunteers of normal body weight underwent 2 study periods of 4 days of diet with or without THL 120 mg t.i.d. On day 5, a HBT was performed. &lt;sup&gt;13&lt;\/sup&gt;CO&lt;sub&gt;2&lt;\/sub&gt; recovery in breath samples was measured over 24\u00a0h by isotope ratio mass spectrometry. &lt;i&gt;Results:&lt;\/i&gt; The peak &lt;sup&gt;13&lt;\/sup&gt;CO&lt;sub&gt;2&lt;\/sub&gt; excretion occurred only after 5\u00a0h in both treatment groups with little difference during the first 4\u00a0h between the groups. THL potently reduced fat digestion and absorption with the most pronounced effect observed after 8\u00a0h: 1.1\u00b1 0.2 vs. 2.3 \u00b1 0.3% dose in control experiments (p &lt; 0.05). The 24-hour cumulative &lt;sup&gt;13&lt;\/sup&gt;CO&lt;sub&gt;2&lt;\/sub&gt; excretion was also significantly reduced by THL: 14.9 \u00b1 2.2 vs. 28.4 \u00b1 4.1% dose in control experiments (p &lt; 0.05). In 6 of the 8 subjects, the cumulative &lt;sup&gt;13&lt;\/sup&gt;CO&lt;sub&gt;2&lt;\/sub&gt; excretion was lower with THL 120\u00a0mg t.i.d. than with placebo. &lt;i&gt;Conclusion:&lt;\/i&gt; The HBT well reflects THL-induced artificial fat maldigestion in healthy volunteers.<\/jats:p>","DOI":"10.1159\/000007808","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:42:49Z","timestamp":1050421369000},"page":"159-163","source":"Crossref","is-referenced-by-count":2,"title":["Induction of Artificial Fat Maldigestion by Tetrahydrolipstatin Assessed by the &lt;sup&gt;13&lt;\/sup&gt;C-Hiolein Breath Test in Healthy Volunteers"],"prefix":"10.1159","volume":"62","author":[{"given":"Hasan","family":"Ashraf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pius","family":"Hildebrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remy","family":"Meier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Beglinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niklaus","family":"Gyr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,9,27]]},"container-title":["Digestion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/7808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/7808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:41:52Z","timestamp":1745469712000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000007808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":0,"journal-issue":{"issue":"2-3","published-online":{"date-parts":[[2000,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000007808","archive":["Portico"],"relation":{},"ISSN":["0012-2823","1421-9867"],"issn-type":[{"type":"print","value":"0012-2823"},{"type":"electronic","value":"1421-9867"}],"subject":[],"published":{"date-parts":[[2000]]}}}