{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:01:24Z","timestamp":1767852084903,"version":"3.49.0"},"reference-count":1,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[1998]]},"abstract":"The aim of the present investigation was to determine which subtypes of the \u03b1<sub>2<\/sub>-adrenoceptors are being expressed in the human pregnant myometrium at term pregnancy. In radioligand binding studies, the specific binding of [<sup>3<\/sup>H]rauwolscine to human myometrial membranes was specific and of high affinity with K<sub>d<\/sub> of 2.8 \u00b1 0.6\u00a0n<i>M<\/i> and B<sub>max<\/sub> of 95 \u00b1 5\u00a0fmol\/mg protein. Results from competition for the binding of [<sup>3<\/sup>H]rauwolscine using subtype-selective ligands, oxymetazoline (\u03b1<sub>2<\/sub>A-subptype), chlorpromazine (\u03b1<sub>2<\/sub>B-subtype) and prazosin (\u03b1<sub>2<\/sub>B-\u03b1<sub>2<\/sub>C-subtype), suggested that the \u03b1<sub>2<\/sub>A- and \u03b1<sub>2<\/sub>B-subtypes are being co-expressed. In order to examine if also the \u03b1<sub>2<\/sub>C-subtype is being expressed we used an optimal concentration of oxymetazoline or chlorpromazine which would block the high-affinity site, equivalent to the \u03b1<sub>2<\/sub>A- and \u03b1<sub>2<\/sub>B-subtype respectively. Competition curves of both oxymetazoline and chlorpromazine still showed a significantly better fit using a two-site model, suggesting that the \u03b1<sub>2<\/sub>C-subtype also is being expressed. The expression of \u03b1<sub>2<\/sub>C-subtype mRNA was confirmed using reverse transcription-polymerase chain reaction on mRNA isolated from myometrial biopsies.In conclusion, our results suggest that all three subtypes of \u03b1<sub>2<\/sub>-adrenoceptors are being coexpressed in the human myometrium at term pregnancy and that \u03b1<sub>2<\/sub>-expression is dominated by the \u03b1<sub>2<\/sub>A-subtype.<\/jats:p>","DOI":"10.1159\/000009944","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:42:49Z","timestamp":1050421369000},"page":"145-150","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of \u03b1<sub>2<\/sub>-Adrenoceptor Subtypes in Pregnant Human Myometrium"],"prefix":"10.1159","volume":"45","author":[{"given":"Per I.","family":"Adolfsson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars Olav","family":"Dahle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00f6ran","family":"Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel P.S.","family":"Svensson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,4,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.18.11012"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/9944","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/9944","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:48:27Z","timestamp":1745470107000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000009944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":1,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1998,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000009944","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998]]}}}