{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:14:13Z","timestamp":1745554453283,"version":"3.40.4"},"reference-count":4,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2000]]},"abstract":"To investigate the mechanism of human uterine smooth muscle relaxation, the activation of Ca<sup>2+<\/sup>-activated K<sup>+<\/sup> channels in cultured myometrial cells obtained from human pregnant myometrium at term by nitric oxide was evaluated at the single cell level using the patch-clamp technique. The open probability of the K<sup>+<\/sup> channel after the addition of 3 \u00d7 10<sup>\u20133<\/sup>\u00a0<i>M<\/i> isosorbide dinitrate, a nitric oxide donor (0.116 \u00b1 0.048) was significantly higher than that before the addition (0.059 \u00b1 0.032; n = 9, p < 0.01). In myometrial cells pretreated with lipopolysaccharide, activation of K<sup>+<\/sup> channels was also noted after the addition of <i>L<\/i>-arginine (10<sup>\u20134<\/sup>\u00a0<i>M<\/i>; open probability increased from 0.179 \u00b1 0.076 to 0.380 \u00b1 0.105, n = 9, p < 0.01: 10<sup>\u20133<\/sup>\u00a0<i>M<\/i>; open probability increased from 0.073 \u00b1 0.050 to 0.242 \u00b1 0.098, n = 12, p < 0.01). Either 10<sup>\u20133<\/sup>\u00a0<i>M<\/i> N-nitro-<i>L<\/i>-arginine-methyl-ester, an inhibitor of nitric oxide synthase, or 10<sup>\u20136<\/sup>\u00a0<i>M<\/i> methylene blue, an inhibitor of guanylate cyclase, abolished activation of the K<sup>+<\/sup> channel by 10<sup>\u20133<\/sup>\u00a0<i>M<\/i> <i>L<\/i>-arginine in pretreated myometrial cells with lipopolysaccharide. Application of 10<sup>\u20133<\/sup>\u00a0<i>M<\/i> <i>L<\/i>-arginine to the intracellular surface of an excised inside-out patch in the myometrial cells pretreated with lipopolysaccharide failed to increase Ca<sup>2+<\/sup>-activated K<sup>+<\/sup> channel activity, suggesting that the activation was mediated by intracellular messengers. These results indicate that nitric oxide should control human myometrial relaxation during pregnancy via activation of Ca<sup>2+<\/sup>-activated K<sup>+<\/sup> channels.<\/jats:p>","DOI":"10.1159\/000010254","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:43:24Z","timestamp":1050421404000},"page":"249-254","source":"Crossref","is-referenced-by-count":11,"title":["Activation of Ca<sup>2+<\/sup>-Activated K<sup>+<\/sup> Channels in Human Myometrium by Nitric Oxide"],"prefix":"10.1159","volume":"49","author":[{"given":"Miho","family":"Shimano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Nakaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rijin","family":"Fukui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaharu","family":"Kamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Hamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhisa","family":"Maeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Aono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,5,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)92405-8"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.4.1899"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)92468-6"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(95)90500-6"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/10254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/10254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:51:53Z","timestamp":1745470313000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000010254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":4,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2000,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000010254","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"type":"print","value":"0378-7346"},{"type":"electronic","value":"1423-002X"}],"subject":[],"published":{"date-parts":[[2000]]}}}