{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:27:00Z","timestamp":1762003620940,"version":"3.40.4"},"reference-count":2,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[1998]]},"abstract":"<jats:p>Sepsis is believed to increase the risk of bilirubin brain toxicity, but the mechanism is not known. Adult male Sprague-Dawley rats were injected intraperitoneally with either 20\u00a0mg\/kg &lt;i&gt;Escherichia coli&lt;\/i&gt; lipopolysaccharide, \u22485 \u00d7 10&lt;sup&gt;9&lt;\/sup&gt;\/kg CFU &lt;i&gt;Listeria monocytogenes&lt;\/i&gt; or vehicle 48\u00a0h prior to sacrifice. Rats were killed with an intraperitoneal injection of pentobarbital. Mitochondrial membrane fractions were produced by homogenization of the brains and differential centrifugation in 0.32\u00a0&lt;i&gt;M&lt;\/i&gt; sucrose. The mitochondrial pellet was resuspended in distilled water and sonicated to rupture the mitochondria. The protein concentration of the suspension was standardized to 2.5\u00a0mg\/ml. Bilirubin oxidation was assayed in a pH 8.2, 0.1 &lt;i&gt;M&lt;\/i&gt; barbital buffer containing 10\u00a0\u00b5&lt;i&gt;M&lt;\/i&gt; bilirubin, 5\u00a0m&lt;i&gt;M&lt;\/i&gt; EDTA, and 500\u00a0U\/ml catalase. Optical density was measured at 440\u00a0nm before and after a 60-min incubation at 37.5\u00b0C. There were no differences between the control, endotoxemic, and septic groups as far as the ability of brain mitochondrial membranes to oxidize bilirubin (bilirubin oxidation rate: 289 \u00b1 11 vs. 295 \u00b1 9 vs. 296 \u00b1 12\u00a0pmol\/min\/mg protein, mean \u00b1 SD). We conclude that endotoxemia or sepsis do not change the ability of brain mitochondrial membranes to oxidize bilirubin. If sepsis truly increases the risk of bilirubin encephalopathy in neonatal jaundice, this is likely to involve other mechanisms.<\/jats:p>","DOI":"10.1159\/000013994","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:43:24Z","timestamp":1050421404000},"page":"340-345","source":"Crossref","is-referenced-by-count":11,"title":["Effects of Endotoxemia and Sepsis on Bilirubin Oxidation by Rat Brain Mitochondrial Membranes"],"prefix":"10.1159","volume":"73","author":[{"given":"Jeffrey W.","family":"Allen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Tommarello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Carcillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thor Willy Ruud","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,4,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0192-0561(94)90094-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199607000-00026"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/13994","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/13994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T05:06:56Z","timestamp":1745471216000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000013994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":2,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1998,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000013994","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[1998]]}}}