{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:13:11Z","timestamp":1790115191751,"version":"4.0.1"},"reference-count":3,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Caries Res"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>This study examined the cultivable microflora before and after stepwise excavation procedures in deep carious lesions in 9 permanent teeth, categorized according to degrees of proximal surface destruction. The final excavation was performed 4\u20136 months after the initial treatment, which included peripheral dentine excavation and removal of the central cariogenic biomass and the superficial necrotic dentine. Dentine colour and consistency were assessed by means of standardized scales before the application of a Ca(OH)&lt;sub&gt;2&lt;\/sub&gt; compound and temporary sealing. Reassessments were performed before and after the final excavation. Microbiological samples of the central demineralized dentine were obtained with a sterile bur before and after the first excavation, as well as before and after the final excavation. After anaerobic cultivation on enriched non\u2013selective tryptic soy agar, 30 colonies from a representative area were identified by standardized biochemical and physiological tests. Before temporary restoration, a yellowish and light brown demineralized soft dentine was typically observed, and gram\u2013positive rods accounted for 70% and lactobacilli for 50% of the total colony\u2013forming units. &lt;i&gt;Lactobacillus casei&lt;\/i&gt; subsp. &lt;i&gt;rhamnosus&lt;\/i&gt; and &lt;i&gt;Actinomyces naeslundii&lt;\/i&gt; dominated the lactobacilli and the other gram\u2013positive rods, respectively. Gram\u2013negative rods were the next most frequent isolates, followed by streptococci, each group accounting for about 20% of the colony\u2013forming units in positive samples. Before the final excavation, which did not cause exposure of the pulp in any of the cases, the retained demineralized dentine had changed into a darker and harder tissue, and the total colony\u2013forming units, as well as the frequency and proportions of lactobacilli were substantially reduced. Gram\u2013negative rods also declined, and the flora was dominated by &lt;i&gt;A. naeslundii&lt;\/i&gt; and various streptococci. In conclusion, the cultivable flora detected following the treatment interval had declined substantially, and the distribution of bacterial species did not represent a typical cariogenic microbiota of deep lesions, confirming the clinical findings of arrested caries progression.<\/jats:p>","DOI":"10.1159\/000016631","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:25:18Z","timestamp":1050427518000},"page":"502-508","source":"Crossref","is-referenced-by-count":150,"title":["Changes in the Cultivable Flora in Deep Carious Lesions following a Stepwise Excavation Procedure"],"prefix":"10.1159","volume":"34","author":[{"given":"L.","family":"Bj\u00f8rndal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2000,11,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000016495"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000016438"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(62)90140-1"}],"container-title":["Caries Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/16631","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/16631","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:45:18Z","timestamp":1745469918000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000016631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":3,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2000,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000016631","archive":["Portico"],"relation":{},"ISSN":["0008-6568","1421-976X"],"issn-type":[{"value":"0008-6568","type":"print"},{"value":"1421-976X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000]]}}}