{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T06:56:43Z","timestamp":1761893803580,"version":"3.40.4"},"reference-count":4,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[1998]]},"abstract":"<jats:p>&lt;b&gt;Background:&lt;\/b&gt; Interferon-\u03b3 (IFN-\u03b3) is used in the treatment of tumor stage mycosis fungoides (MF), whereas its mechanism is still unknown. We have previously shown that treatment with intralesional IFN-\u03b3 induced tumor regression. &lt;b&gt;Objective:&lt;\/b&gt; To explore the possibility that IFN-\u03b3 may alter the cytokine expression by tumor cells, we studied cytokine gene expression in a tumor nodule of MF. &lt;b&gt;Methods:&lt;\/b&gt; By using the reverse transcriptase-polymerase chain reaction, Th1- and Th2-type cytokine mRNA expression was examined before and after intralesional IFN-\u03b3 therapy. Additionally, we examined bcl-2 protein expression on tumor cells. &lt;b&gt;Results: &lt;\/b&gt;We found weak mRNA expression of interleukin-4 (IL-4) and IL-5, and strong expression of IL-6, IL-10 and IFN-\u03b3 before therapy. After successful treatment of intralesional IFN-\u03b3, mRNA expression of IL-5, IL-6 and IL-10 were significantly reduced and IL-2 mRNA was mildly induced. Culture for 24 h of tumor cells with IFN-\u03b3 showed upregulation of IL-2 mRNA and downregulation of IL-6 mRNA expression. bcl-2 expression was significantly decreased after successful intralesional IFN-\u03b3 therapy, photochemotherapy (PUVA) and radiation therapy. &lt;b&gt;Conclusion:&lt;\/b&gt; These data suggest that IFN-\u03b3 induces tumor regression by affecting cytokine gene expression in MF tumor lesions. The reduced bcl-2 expression did not seem to be induced by a direct immunological affect of IFN-\u03b3, but to represent a nonspecific result of tumor regression after successful treatment.<\/jats:p>","DOI":"10.1159\/000017921","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:25:18Z","timestamp":1050427518000},"page":"283-287","source":"Crossref","is-referenced-by-count":7,"title":["Alteration of Cytokine Genes and bcl-2 Expression following Immunotherapy with Intralesional IFN-\u03b3 in a Patient with Tumor-Stage Mycosis fungoides"],"prefix":"10.1159","volume":"196","author":[{"given":"T.","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Katayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nishioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,5,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/348334a0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.7.1.145"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.129.4.433"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.131.8.909"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/17921","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/17921","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:53:18Z","timestamp":1745470398000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000017921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":4,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1998,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000017921","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"type":"print","value":"1018-8665"},{"type":"electronic","value":"1421-9832"}],"subject":[],"published":{"date-parts":[[1998]]}}}