{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:13:17Z","timestamp":1745554397226,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"4-5","license":[{"start":{"date-parts":[[2000,7,31]],"date-time":"2000-07-31T00:00:00Z","timestamp":965001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,7,31]],"date-time":"2000-07-31T00:00:00Z","timestamp":965001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<jats:p>Sublytic complement injury to glomerular mesangial cells, mediated by the terminal membrane attack complex of complement (C5b-9), is a potential initiating mechanism in IgA nephropathy. Sublytic complement injury has been reported to result in the production of a variety of pro-inflammatory molecules and growth factors, including many regulated by the transcription factor NF-\u03baB. To determine the importance of complement injury in the pro-inflammatory signalling which occurs in IgA nephropathy, we investigated NF-\u03baB activation following sublytic complement injury to cultured rat glomerular mesangial cells (RMCs). A sublytic dose of rabbit anti-Thy 1.1 (THY) serum and normal human serum was selected based upon flow cytometry, chromium-release assay, and induction of superoxide production. No significant C5b-9-induced NF-\u03baB activation was detected by electrophoretic mobility shift assays, luciferase activity of RMCs transfected with a NF-\u03baB-driven luciferase reporter construct, nor by Northern blots for the NF-\u03baB-responsive mRNA species monocyte chemoattractant protein-1 or I\u03baB\u03b1. Furthermore, measurements of &lt;sup&gt;3&lt;\/sup&gt;H incorporation following sublytic complement injury showed inhibition of mesangial cell mitogenesis in comparison to the heat-inactivated serum treatment and to THY alone. The results of this study suggest that sublytic complement injury to RMC does not directly activate NF-\u03baB nor induce mesangial cell proliferation in mesangial cells. Other mechanisms such as IgA immune complex formation must be required to produce these events in IgA nephropathy.<\/jats:p>","DOI":"10.1159\/000020681","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:26:45Z","timestamp":1050427605000},"page":"291-298","source":"Crossref","is-referenced-by-count":4,"title":["Sublytic Complement Injury Does Not Activate NF-\u03baB, or Induce Mitogenesis in Rat Mesangial Cells"],"prefix":"10.1159","volume":"8","author":[{"given":"S.J.","family":"Mudge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.L.","family":"McRae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.B.","family":"Auwardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.F.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.-G.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.A.","family":"Power","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,7,31]]},"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/20681","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/20681","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:33:47Z","timestamp":1745469227000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000020681"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,7,31]]},"references-count":0,"journal-issue":{"issue":"4-5","published-online":{"date-parts":[[2000,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000020681","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"type":"electronic","value":"1660-2129"}],"subject":[],"published":{"date-parts":[[2000,7,31]]}}}