{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:13:45Z","timestamp":1745554425986,"version":"3.40.4"},"reference-count":2,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathophysiol Haemos Thromb"],"published-print":{"date-parts":[[1998]]},"abstract":"<jats:p>Irradiation in the presence of 1\u00a0\u00b5M methylene blue (MB) inactivates enveloped viruses in human plasma. This method is a useful tool to enhance the safety of human fresh-frozen plasma in blood donor banks. Via a photooxidative mechanism, viral structures as well as plasma proteins, especially fibrinogen, can be damaged. We investigated the effect of MB\/light-induced photooxidative modification on the structure of fibrinogen and its function in the interaction with platelets in the concentration range of 1\u201350\u00a0\u00b5M MB. Densitometric scanning of reduced SDS-PAGE showed a decrease in the A\u03b1-subunit of fibrinogen as well as an increase in high-molecular-weight (HMW) aggregates with increasing MB concentrations. The HMW aggregates may be due to covalent crosslinking of single A\u03b1-subunits via photooxidation induced by MB\/light treatment. Fibrinogen treated with high doses of MB (50\u00a0\u00b5M) showed a weaker binding to the fibrinogen receptor (GP IIb\/IIIa) on the platelet surface and a decrease in platelet aggregation after stimulation with ADP and photooxidized fibrinogen. These effects were not detected in fibrinogen isolated from virus-inactivated plasma (1\u00a0\u00b5M MB). This was in agreement with normal binding of fibrinogen to GP IIb\/IIIa on platelets.<\/jats:p>","DOI":"10.1159\/000022379","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:26:45Z","timestamp":1050427605000},"page":"17-24","source":"Crossref","is-referenced-by-count":1,"title":["High Doses of Methylene Blue\/Light Treatment Crosslink the A-Alpha-Subunit of Fibrinogen: Influence of This Photooxidization on Fibrinogen Binding to Platelets"],"prefix":"10.1159","volume":"28","author":[{"given":"M.","family":"Lorenz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Jablonka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Vigh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.M.","family":"Kirchmaier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,1,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(73)90196-2"}],"container-title":["Pathophysiology of Haemostasis and Thrombosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/22379","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/22379","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:42:48Z","timestamp":1745469768000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000022379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":2,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1998,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000022379","archive":["Portico"],"relation":{},"ISSN":["1424-8832","1424-8840"],"issn-type":[{"type":"print","value":"1424-8832"},{"type":"electronic","value":"1424-8840"}],"subject":[],"published":{"date-parts":[[1998]]}}}