{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:45:03Z","timestamp":1757702703548,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Res Paediatr"],"published-print":{"date-parts":[[1998]]},"abstract":"Receptors for insulin-like growth factors (IGF-I and IGF-II) are expressed in mammalian intestinal epithelium. No information on the presence of IGF receptors in intestinal endocrine cells is available. We tested for IGF-I receptors the endocrine cell line STC-1, which synthesizes and processes cholecystokinin (CCK) among other peptides, and assessed the effects of IGF-I on cell growth and CCK content. Cell monolayers in serum-free culture medium specifically bound [<sup>125<\/sup>I]IGF-I. Scatchard analysis was consistent with a single class of high affinity binding sites (K<sub>D<\/sub> = 0.91\u00a0n<i>M<\/i>; B<sub>max<\/sub> = 4,700 sites\/cell). In competitive binding assays, unlabeled IGF-I, IGF-II and insulin displaced in a dose-dependent manner [<sup>125<\/sup>I]IGF-I binding with the following potencies (K<sub>I<\/sub>): IGF-I (0.74\u00a0n<i>M<\/i>) > IGF-II (3\u00a0n<i>M<\/i>) >> insulin (1\u00a0\u00b5<i>M<\/i>). Affinity cross-linking with [<sup>125<\/sup>I]IGF-I using disuccinimidyl suberate and SDS-PAGE under reducing conditions yielded a polypeptide band with apparent M<sub>r<\/sub> 130,000, consistent with the \u03b1-subunit of the IGF-I receptor. IGF-I and IGF-II (0.3\u201330\u00a0n<i>M<\/i>) dose-dependently stimulated [<sup>3<\/sup>H]thymidine incorporation, with a maximal response of 110% above basal. IGF-II was approximately 10-fold less potent than IGF-I, suggesting a mediation through IGF-I receptors. In addition, the numbers of cells treated with 3\u00a0n<i>M<\/i> IGF-I amounted to 116, 130 and 159% of control values after 1, 2 and 4 days of incubation, respectively (p < 0.05). A significant increase in the cell CCK contents was observed after a 48-hour exposure to 3 or 30\u00a0n<i>M<\/i> IGF-I. These results demonstrate IGF-I receptor expression by the enteroendocrine cell line STC-1. IGF-I stimulates proliferation in short-term experiments, and increases intracellular levels of CCK.<\/jats:p>","DOI":"10.1159\/000023271","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:26:45Z","timestamp":1050427605000},"page":"183-189","source":"Crossref","is-referenced-by-count":1,"title":["Insulin-Like Growth Factor I Receptors Are Expressed by the Enteroendocrine Cell Line STC-1: Relationship with Proliferation and Cholecystokinin Expression"],"prefix":"10.1159","volume":"50","author":[{"given":"Fei","family":"Ye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Marie","family":"Chevrier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Langlois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Claude","family":"Cuber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose M.","family":"Saez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Alain","family":"Chayvialle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Abello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,10,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1210\/er.16.1.3"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.61.1.307"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1210\/jc.75.2.609"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(94)90228-3"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)90642-0"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00371-8"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.2.885"}],"container-title":["Hormone Research in Paediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:49:21Z","timestamp":1745470161000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000023271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":7,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1998,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000023271","archive":["Portico"],"relation":{},"ISSN":["1663-2818","1663-2826"],"issn-type":[{"type":"print","value":"1663-2818"},{"type":"electronic","value":"1663-2826"}],"subject":[],"published":{"date-parts":[[1998]]}}}