{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:34:35Z","timestamp":1770910475304,"version":"3.50.1"},"reference-count":3,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[1997,12,22]],"date-time":"1997-12-22T00:00:00Z","timestamp":882748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1997,12,22]],"date-time":"1997-12-22T00:00:00Z","timestamp":882748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[1998]]},"abstract":"<b>Background:<\/b> Mast cells (MC) are involved in a wide spectrum of disorders characterized by neovascularization and fibroproliferation. We and others recently reported that human MC are a source of basic fibroblast growth factor (b FGF-2), a potent angiogenic and mitogenic polypeptide, in several disease conditions, such as chronic inflammation, hemangioma, and benign cutaneous mastocytosis. These findings suggest that FGF-2 may be an important mediator of cell proliferation and angiogenesis associated with MC. Since MC are heterogeneous across species, it is unknown whether FGF-2 expression is a feature common to all MC, or whether FGF-2 expression by MC can be regulated. We therefore examined FGF-2 expression by MC in mouse tissue and MC lines. <b>Methods: <\/b>Immunostaining, RT-PCR, ELISA, immunoblot and Northern blot analyses were employed to study four murine MC lines for FGF-2 expression and its regulation by transforming growth factor-\u03b2 (TGF-\u03b2), stem cell factor (SCF), and tumor necrosis factor-\u03b1 (TNF-\u03b1). <b>Results: <\/b>Mouse tissue MC and three of four murine MC lines (CFTL-12, CFTL-15, ABFTL-3) express FGF-2 as judged by immunostaining, ELISA, Western blot and Northern blot analyses, and reverse transcription-polymerase chain reaction. While TNF-\u03b1 appeared to downregulate FGF-2 mRNA levels, treatment with SCF or TGF-\u03b2 resulted in an increase in the expression of FGF-2 at mRNA level which can be attenuated by TNF-\u03b1. However, the concurrent increase in FGF-2 protein was negligible, possibly due to immaturity of these cell lines. <b>Conclusion:<\/b> Expression of FGF-2 may be a ubiquitous feature of MC in other species in addition to humans, and can be selectively regulated by SCF, TGF-\u03b2 and TNF-\u03b1.<\/jats:p>","DOI":"10.1159\/000023829","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"47-54","source":"Crossref","is-referenced-by-count":55,"title":["Synthesis of Basic Fibroblast Growth Factor by Murine Mast CellsRegulation by Transforming Growth Factor Beta, Tumor Necrosis Factor Alpha, and Stem Cell Factor"],"prefix":"10.1159","volume":"115","author":[{"given":"Zhenhong","family":"Qu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaona","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Proochista","family":"Ahmadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Stenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice M.","family":"Liebler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anh-Chi","family":"Le","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen R.","family":"Planck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James T.","family":"Rosenbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1997,12,22]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1987.9999"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90326-T"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/346274a0"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23829","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23829","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:52:11Z","timestamp":1745470331000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000023829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12,22]]},"references-count":3,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1998,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000023829","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12,22]]}}}