{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T23:42:32Z","timestamp":1747698152029,"version":"3.40.4"},"reference-count":1,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[1998]]},"abstract":"<jats:p>We have previously reported that purified Shiga\u2013like toxins (SLT), SLT\u2013I and SLT\u2013II coupled with liposomes induced a substantial amount of anti\u2013SLT\u2013I and anti\u2013SLT\u2013II IgG antibody production, respectively, in mice. The levels of anti\u2013SLT antibody in the sera of SLT\u2013liposome\u2013immune mice correlated well with the protection against subsequent challenge with SLT. In this study, mice were immunized intraperitoneally with the mixture of SLT\u2013I\u2013liposome and SLT\u2013II\u2013liposome and protection against oral infection with cytotoxin\u2013producing Escherichia coli O157:H7 was evaluated. All of the mice that received immunization with the mixture of SLT\u2013I\u2013liposome and SLT\u2013II\u2013liposome were protected against subsequent intravenous challenge with 10 LD&lt;sub&gt;50&lt;\/sub&gt; of either SLT\u2013I or SLT\u2013II. Eight weeks after primary immunization, mice were inoculated intragastrically with 10&lt;sup&gt;9&lt;\/sup&gt; CFU of E. coli O157:H7 strain 96\u201360. All SLT\u2013liposome\u2013immune mice tested survived without any apparent symptom while control mice died within 5 days. In addition, as shown by other antigen\u2013liposome conjugates, SLT\u2013liposome induced undetectable anti\u2013SLT IgE antibody production while they induced substantial amounts of anti\u2013SLT IgG antibodies. These results suggest that SLT\u2013liposome conjugate may serve as a candidate vaccine that induces protection against cytotoxin\u2013producing E. coli infection.<\/jats:p>","DOI":"10.1159\/000023961","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"313-317","source":"Crossref","is-referenced-by-count":13,"title":["Induction of Protection against Oral  Infection with Cytotoxin\u2013Producing  Escherichia coli O157:H7 in Mice by  Shiga\u2013like Toxin\u2013Liposome Conjugate"],"prefix":"10.1159","volume":"116","author":[{"given":"Tadashi","family":"Fukuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takako","family":"Kimiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motohide","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshichika","family":"Arakawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Ami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuriko","family":"Suzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seishiro","family":"Naito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuko","family":"Horino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyo","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachihiro","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Gondaira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-ichi","family":"Sugiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahito","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouichi","family":"Nishinohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsutoshi","family":"Komuro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Uchida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,8,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1097(88)90402-8"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23961","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23961","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:53:20Z","timestamp":1745470400000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000023961"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":1,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1998,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000023961","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[1998]]}}}