{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:38:48Z","timestamp":1761057528088,"version":"3.40.4"},"reference-count":3,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[1998]]},"abstract":"<jats:p>&lt;b&gt;Background:&lt;\/b&gt; Pollen from oilseed rape (OSR), Brassica napus, an increasingly cultivated oilplant from the Brassicaceae, has been recognized as a potential cause of allergic sensitization. Allergens have been hardly investigated. &lt;b&gt;Methods:&lt;\/b&gt; We characterized IgE binding proteins in OSR pollen by immunoblot, immunoblot inhibition and specific monoclonal antibodies using sera from 89 patients sensitized to OSR. &lt;b&gt;Results:&lt;\/b&gt; Two low\u2013molecular\u2013weight allergens of 6\/8 kD and 14 kD as well as a high molecular\u2013weight cluster (27\u201369 kD) comprising six cross\u2013reactive peptides could be identified. The three allergens were recognized by 50, 34 and 80% of patients, respectively. Immunoblot IgE binding to OSR could be totally inhibited by rye pollen and moderately by birch pollen (6\/8 and 14 kD) while mugwort had little effect. An anti\u2013profilin\u2013specific monoclonal antibody bound specifically to a 14\u2013kD protein in OSR. Binding to the 6\/8\u2013kD rape allergen could be effectively inhibited by rAln g 2, a calcium\u2013binding protein from alder. Periodate treatment led to a significant reduction in IgE binding to the 27 to 69\u2013kD OSR allergens indicating that carbohydrate determinants are involved in IgE binding. OSR proteins were capable to quench IgE binding to timothy grass pollen proteins of \u226560 kD suggesting that grass pollen group 4 allergens cross\u2013react with the 27 to 69\u2013kD cluster in OSR. &lt;b&gt;Conclusions:&lt;\/b&gt; The data demonstrate that OSR pollen is allergenic and indicate that the identified allergens represent cross\u2013reacting homologues of well\u2013known pollen allergens, i.e. calcium\u2013binding proteins, profilins, and high\u2013molecular\u2013weight glycoproteins. Via cross\u2013reactivity, exposure to OSR pollen may be a prolonging and aggravating factor in underlying birch and grass pollen allergy.<\/jats:p>","DOI":"10.1159\/000023996","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"105-112","source":"Crossref","is-referenced-by-count":28,"title":["Identification of Allergens in Oilseed Rape (Brassica napus) Pollen"],"prefix":"10.1159","volume":"117","author":[{"given":"M.","family":"Focke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Hemmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Hayek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"G\u00f6tz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Jarisch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,10,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.2.377"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00772.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(87)90170-4"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/23996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:53:33Z","timestamp":1745470413000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000023996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":3,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1998,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000023996","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[1998]]}}}