{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T13:45:34Z","timestamp":1760622334797,"version":"3.40.4"},"reference-count":1,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[1998]]},"abstract":"<jats:p>&lt;b&gt;Background:&lt;\/b&gt; Brochial asthma is characterized by infiltration of inflammatory cells such as lymphocytes and eosinophils. Theophylline is one of the most widely used drugs in the therapy of bronchial asthma, and phosphodiesterase (PDE) inhibition is thought to be an important mechanism of its anti\u2013inflammatory actions. However, the detailed effects of PDE inhibition on eosinophils still remain unclear. &lt;b&gt;Methods:&lt;\/b&gt; Eosinophils in peripheral blood obtained from normal subjects and patients with mild off\u2013season allergic rhinitis were purified using CD16 negative selection. The following effects of theophylline (nonselective PDE inhibitor), KF19514 (selective PDE IV inhibitor), mirlinone (selective PDE III inhibitor), procaterol (\u03b22\u2013adrenoceptor agonist) and N&lt;sup&gt;6&lt;\/sup&gt;, 2\u2032\u2013O\u2013dibutyryladenosine 3\u20325\u2032\u2013cyclic monophosphate (dB\u2013cAMP; AMP analogue) on eosinophils were examined: (1) survival in the presence of interleukin\u20135, (2) degranulation by granulocyte\/macrophage colony\u2013stimulating factor (GM\u2013CSF) or platelet\u2013activating factor (PAF), (3) CD11b expression under GM\u2013CSF or PAF stimulation and (4) intracellular cAMP level. &lt;b&gt;Results:&lt;\/b&gt; Eosinophil survival was inhibited by theophilline, KF19514 or procaterol. GM\u2013CSF\u2013 or PAF\u2013induced degranulation was inhibited by theophylline, KF19514, procaterol or dB\u2013cAMP. CD11b up\u2013regulation by PAF was inhibited by theophylline, KF19514 or dB\u2013cAMP, while GM\u2013CSF\u2013stimulated CD11b up\u2013regulation was not significantly inhibited by any of the drugs tested. The levels of intracellular cAMP were increased by theophylline, KF19514 and procaterol. &lt;b&gt;Conclusions:&lt;\/b&gt; Intracellular cAMP is an important factor in the regulation of eosinophil biological functions. PDE IV inhibitors and \u03b2&lt;sub&gt;2&lt;\/sub&gt;\u2013agonists are suggested to be useful for the treatment of bronchial asthma through inhibition of eosinophil effector function.<\/jats:p>","DOI":"10.1159\/000024001","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"138-145","source":"Crossref","is-referenced-by-count":25,"title":["Effects of Intracellular Cyclic AMP Modulators on Human Eosinophil Survival, Degranulation and CD11b Expression"],"prefix":"10.1159","volume":"117","author":[{"given":"T.","family":"Momose","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Okubo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Horie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Isobe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sekiguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1998,10,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90519-N"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:53:42Z","timestamp":1745470422000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998]]},"references-count":1,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1998,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024001","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[1998]]}}}