{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T18:02:33Z","timestamp":1782928953005,"version":"3.54.5"},"reference-count":1,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[1999]]},"abstract":"<jats:p>&lt;b&gt;Background: &lt;\/b&gt;To determine whether antigen challenge is associated with increased accumulation of leukocytes in the lungs, the pulmonary accumulation of &lt;sup&gt;111&lt;\/sup&gt;In\u2013labelled neutrophils and eosinophils was monitored by gamma scintigraphy. &lt;b&gt;Methods: &lt;\/b&gt;Guinea\u2013pigs were sensitized with ovalbumin (OA) 14\u201321 days before challenge with aerosolized OA (10 mg\/ml) for 2 min, and protected against fatal anaphylaxis by mepyramine (30 mg\/kg). Comparisons were made with OA\u2013sensitized guinea\u2013pigs challenged with saline. 5 or 24 h following the OA challenge, guinea\u2013pigs were anaesthetized and the jugular vein and right carotid artery were cannulated, with the contralateral artery tied off. 2MBq &lt;sup&gt;99m&lt;\/sup&gt;Tc macroaggregated albumin (MAA) was injected intravenously to create a pulmonary perfusion image as a template for the lungs. &lt;sup&gt;111&lt;\/sup&gt;In\u2013labelled neutrophils or eosinophils were then injected via the carotid artery and gamma scintigraphic images obtained. Activity in the lung region of each animal was determined by superimposing the &lt;sup&gt;99m&lt;\/sup&gt;TcMAA image of the lungs on the whole body image. &lt;b&gt;Results: &lt;\/b&gt;A significant increase in activitiy (p&lt;0.05) in the lung region was observed after injection of &lt;sup&gt;111&lt;\/sup&gt;In\u2013labelled neutrophils at 5 h after the OA challenge compared with saline challenge. 24 h after OA challenge there was a significant increase in activity (p&lt;0.05) in the lung region after injection of &lt;sup&gt;111&lt;\/sup&gt;In\u2013labelled eosinophils, but no change in activity after injection of labelled neutrophils compared with the saline\u2013challenged animals. &lt;b&gt;Conclusion: &lt;\/b&gt;This technique models the migration of leukocyte to the lungs seen in guinea\u2013pigs in our previous studies, namely an eosinophilia at 24 h and a neutrophilia at 5 h after OA challenge. It will therefore be useful for investigating anti\u2013inflammatory drugs on the airways.<\/jats:p>","DOI":"10.1159\/000024031","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"51-58","source":"Crossref","is-referenced-by-count":6,"title":["&lt;sup&gt;111&lt;\/sup&gt;In\u2013Labelled Leukocyte Migration to the Lungs of Ovalbumin\u2013Sensitized Guinea\u2013Pigs after Aerosol Challenge with Ovalbumin Monitored by Gamma Scintigraphy"],"prefix":"10.1159","volume":"118","author":[{"given":"Christine A.","family":"Lewis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon J.","family":"Warren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth J.","family":"Broadley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[1999,1,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(90)94159-U"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24031","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:54:09Z","timestamp":1745470449000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999]]},"references-count":1,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1999,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024031","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999]]}}}