{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T23:42:31Z","timestamp":1747698151421,"version":"3.40.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[1999]]},"abstract":"<b>Background:<\/b> We have previously reported that ovalbumin (OVA) coupled with liposome via glutaraldehyde (GA) induced OVA\u2013specific\u2013 and IgE\u2013selective unresponsiveness in mice. <b>Methods:<\/b> In this study, OVA\u2013liposome conjugates were made using four different coupling protocols: via GA, N\u2013(6\u2013maleimidocaproyloxy) succinimide (EMCS), disuccinimidyl suberate (DSS) and N\u2013succimidyl\u20133(2\u2013pyridyldithio)propionate (SPDP) and the induction of antigen\u2013specific IgG and IgE antibody production was investigated for each. In addition, antigen\u2013specific cytokine production by spleen cells of mice immunized either with OVA\u2013liposome or with OVA adsorbed with aluminum hydroxide was investigated. <b>Results:<\/b> OVA\u2013liposome conjugates coupled via GA or DSS did not induce anti\u2013OVA IgE antibody production but induced substantial anti\u2013OVA IgG antibody production. On the other hand, the induction of anti\u2013OVA IgE unresponsiveness by OVA\u2013liposome conjugates coupled via EMCS or SPDP was incomplete. The amount of interleukin 4 (IL\u20134) produced by spleen cells stimulated in vitro with OVA correlated well with anti\u2013OVA IgE antibody production in donor mice. However, the production of no other cytokine, i.e., IL\u20132, IL\u20135, IL\u201310 or interferon\u2013\u03b3, was correlated with in vivo IgE antibody production. <b>Conclusion:<\/b> OVA\u2013liposome coupled via GA or DSS induced complete suppression of anti\u2013OVA IgE production. The results in this study further suggest that the regulation of IgE antibody production does not neccessarily correlate with so\u2013called Th1 cytokine production.<\/jats:p>","DOI":"10.1159\/000024268","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"199-208","source":"Crossref","is-referenced-by-count":12,"title":["Antigen\u2013Specific, IgE\u2013Selective Unresponsiveness Induced by Antigen\u2013Liposome Conjugates"],"prefix":"10.1159","volume":"120","author":[{"given":"Yoshio","family":"Nakano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahito","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouichi","family":"Nishinohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusaku","family":"Takita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seishiro","family":"Naito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuko","family":"Horino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maiko","family":"Taneichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Ami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuriko","family":"Suzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsutoshi","family":"Komuro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Uchida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,11,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199602083340602"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90046-N"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1006\/immu.1994.1023"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1006\/immu.1994.1022"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000023947"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000023961"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.7.1.145"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(96)80606-2"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24268","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:56:39Z","timestamp":1745470599000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999]]},"references-count":8,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1999,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024268","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[1999]]}}}