{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:04:03Z","timestamp":1759939443966,"version":"3.40.4"},"reference-count":12,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>The effect of two Ca&lt;sup&gt;2+&lt;\/sup&gt; ATPase inhibitors, cyclopiazonic acid (CPA) and 2,5-di-(tert-butyl)-1,4-hydroquinone (DTBHQ), on the release of MCP-1 from bone marrow-derived mast cells (BMMCs) were investigated. CPA and DTBHQ increased the intracellular free Ca&lt;sup&gt;2+&lt;\/sup&gt; concentration ([Ca&lt;sup&gt;2+&lt;\/sup&gt;]&lt;sub&gt;i&lt;\/sub&gt;) and induced MCP-1 release in a dose-dependent manner. These Ca&lt;sup&gt;2+&lt;\/sup&gt; ATPase inhibitors induced MCP-1 release in the absence of phorbol ester, in contrast to their induction of TNF-\u03b1. MCP-1 release reached a maximum at 6\u20139\u00a0h. It was inhibited by treatment with actinomycin D, the immunosuppressant cyclosporin A, and the cytosolic Ca&lt;sup&gt;2+&lt;\/sup&gt; chelator BAPTA-AM. Furthermore, RT-PCR showed a time-dependent increase of MCP-1 mRNA. Thus MCP-1 release seems to depend on Ca&lt;sup&gt;2+&lt;\/sup&gt;-dependent transcriptional activation. MCP-1 release was dose-dependently inhibited by the p38 MAP kinase inhibitor SB202190, but not by the p44\/42 MAP kinase inhibitor PD98059. Therefore, transcriptional activation of MCP-1 production and its release seem to be dependent on the nuclear factor of activated T cells and p38 MAP kinase activation. This is the first report to show the regulation of MCP-1 production in BMMCs.<\/jats:p>","DOI":"10.1159\/000024295","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"34-43","source":"Crossref","is-referenced-by-count":15,"title":["Effect of Ca&lt;sup&gt;2+&lt;\/sup&gt; ATPase Inhibitors on MCP-1 Release from Bone Marrow-Derived Mast Cells and the Involvement of p38 MAP Kinase Activation"],"prefix":"10.1159","volume":"121","author":[{"given":"R.","family":"Teshima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Onose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Okunuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Sawada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,2,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90176-A"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.10.1.411"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/33340"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/29788"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/19546"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/339064a0"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/31804"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/43072"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/31960"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90007-8"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.26.16415"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.20.13397"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:57:05Z","timestamp":1745470625000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":12,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2000,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024295","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2000]]}}}