{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T16:54:26Z","timestamp":1760547266593,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>Recent crystal structure analysis of HIV-1 gp41 revealed that two domains (N- and C-domains) on gp41 play an important role in mediating membrane fusion and HIV-1 entry. The experimental evidence that gp41 by N-domain bound the potential receptor protein P45 could help to understand the mechanism of HIV entry. A recombinant soluble gp41 (rsgp41: Env aa539\u2013684) could bind to P45 in the affinity capillary electrophoresis analysis and the surface plasmon resonance assay. In a blockade assay, peptide P1 (Env aa583\u2013599) could inhibit interaction between rsgp41 and P45, while a control peptide could not. Direct binding of rsgp41, rgp41DP (aa567\u2013648), P1 peptide and (P1)&lt;sub&gt;2&lt;\/sub&gt; peptide [(aa586\u2013596)&lt;sub&gt;2&lt;\/sub&gt;] to P45 was examined in an ELISA assay. Rsgp41 bound the potential receptor protein P45 strongly, while rgp41DP and P1 as well as (P1)&lt;sub&gt;2&lt;\/sub&gt; could all weakly bind to P45, indicating that gp41 by N-domain weakly binds P45 and the region RILAVERYLKD located in the N-domain is defined as the binding site for P45 binding.<\/jats:p>","DOI":"10.1159\/000024325","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"253-257","source":"Crossref","is-referenced-by-count":7,"title":["HIV-1 gp41 by N-Domain Binds the Potential Receptor Protein P45"],"prefix":"10.1159","volume":"121","author":[{"given":"Yi","family":"Xiao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weicheng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred P.","family":"Dierich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Hua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,3,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/381667a0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/387426a0"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80205-6"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(94)90092-2"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(93)90134-W"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(92)90011-C"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81430-0"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24325","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:57:35Z","timestamp":1745470655000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":7,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2000,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024325","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2000]]}}}