{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T12:42:08Z","timestamp":1767703328068,"version":"3.40.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; We established a T cell line, STO-5, which constitutively produced monocyte\/macrophage chemotactic activity via human T cell lymphoma-leukemia-virus-induced transformation of normal human T cells. &lt;i&gt;Methods:&lt;\/i&gt; We isolated and purified a lactose-binding protein, MCF-pl5-L (MW of about 50 kD, pl of about 5) from a conditioned medium of STO-5. By using highly purified MCF-pl5-L, its biological and physicochemical properties were elucidated in comparison with C5a and MCP-1. &lt;i&gt;Results:&lt;\/i&gt; MCF-pl5-L exhibited an evident dose-dependent monocyte chemotactic activity (MCA). MCF-pl5-L had no or little affinity for heparin unlike chemokines such as MCP-1. We further found that MCF-pl5-L exhibited potent chemotactic activity not only for monocytes but also for alveolar macrophages. In contrast, C5a and MCP-1 failed to show evident chemotactic activity for alveolar macrophages though they did show MCA. MCF-pl5-L failed to exhibit evident eosinophil and neutrophil chemotactic activities, indicating its chemotactic activity is selective for monocytes\/macrophages. Regarding the biological functions of MCF-pl5-L other than MCA and chemotactic activity for alveolar macrophages, we found that MCF-pl5-L but not C5a and MCP-1 could prolong the life span of alveolar macrophages, probably by inhibiting apoptosis of macrophages, and stimulate the production of TNF-\u03b1 from macrophages. &lt;i&gt;Conclusions:&lt;\/i&gt; These results suggest that MCF-pl5-L plays a role as an immune modulator for monocytes\/macrophages in the site.<\/jats:p>","DOI":"10.1159\/000024360","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"66-75","source":"Crossref","is-referenced-by-count":4,"title":["Isolation of a Lactose-Binding Protein with Monocyte\/Macrophage Chemotactic Activity"],"prefix":"10.1159","volume":"122","author":[{"given":"Tohru","family":"Yamanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Saita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Kawano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuhiro","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotsugu","family":"Kohrogi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moritaka","family":"Suga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Ando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuomi","family":"Hirashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,5,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/347669a0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.27.16976"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(80)90211-2"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960328)66:1<124::AID-IJC21>3.0.CO;2-C"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.13.6737"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/378736a0"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0954-6111(98)90002-6"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0954-6111(99)90077-X"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24360","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:57:51Z","timestamp":1745470671000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":8,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2000,5,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024360","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2000]]}}}