{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T05:40:07Z","timestamp":1745473207644,"version":"3.40.4"},"reference-count":3,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2000]]},"abstract":"<i>Background:<\/i> Since eosinophils are implicated in asthma pathogenesis, we investigated whether these cells were activated in severe asthma. <i>Methods:<\/i> Twenty-six asthmatics with different clinical responses to oral corticosteroid (CS), i.e. sensitive [change in forced expiratory volume in 1\u00a0s (\u0394FEV<sub>1<\/sub>) \u2265 25% after oral methylprednisolone, 40\u00a0mg daily, for 14 days, n = 7], resistant (\u0394FEV<sub>1<\/sub> \u2264 15%, n = 9) and dependent (\u2265 20\u00a0mg oral prednisone daily for acceptable asthma control, n = 10), were studied. <i>Results:<\/i> Calcium ionophore-induced leukotriene (LT) C<sub>4<\/sub> release of purified blood eosinophils was similar in the three groups. Cell incubation with granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced ionophore-induced LTC<sub>4<\/sub> release, and this effect was higher in CS-sensitive (5-fold) than in CS-resistant subjects (1.7-fold) (p = 0.02). CS treatment decreased blood eosinophil counts in these two groups of subjects (p \u2264 0.02) and decreased GM-CSF-enhanced LTC<sub>4<\/sub> release in CS-sensitive asthmatics only (p = 0.04). In contrast, despite a high mean daily dose of oral CS (35 \u00b1 8\u00a0mg), blood counts of eosinophils from CS-dependent subjects were higher (p = 0.03) and GM-CSF enhancement of LTC<sub>4<\/sub> release was greater (2.8-fold) than in CS-sensitive (2.1-fold) and CS-resistant (1.7-fold) subjects (p = 0.04). Interestingly, serum from CS-resistant subjects reduced GM-CSF enhancement of LTC<sub>4<\/sub> release by eosinophils of CS-sensitive asthmatics (p = 0.001). <i>Conclusions:<\/i> Eosinophils from CS-dependent asthmatics have an impaired response to CS, whereas serum from CS-resistant subjects contains an inhibitor of eosinophil response to GM-CSF.<\/jats:p>","DOI":"10.1159\/000024398","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T09:24:25Z","timestamp":1050398665000},"page":"200-208","source":"Crossref","is-referenced-by-count":0,"title":["Eosinophil Activation Status and Corticosteroid Responsiveness in Severe Asthma"],"prefix":"10.1159","volume":"122","author":[{"given":"M.","family":"Boss\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Audette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Laflamme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ferland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.P.","family":"Boulet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Laviolette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,7,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.1.33"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90351-7"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)90127-9"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24398","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/24398","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:58:00Z","timestamp":1745470680000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000024398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":3,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2000,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000024398","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2000]]}}}