{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:49:20Z","timestamp":1757778560728,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Vasc Res"],"published-print":{"date-parts":[[1999]]},"abstract":"The inhibition by loop diuretics of K efflux (tracer <sup>86<\/sup>Rb) from the rat femoral arterial smooth muscle was measured in normotension and in DOCA-salt hypertension. The sensitivity sequence (bumetanide > piretanide > furosemide) was the characteristic pharmacological profile of (Na+K+Cl) cotransport. In hypertension, cotransport activity was 46% greater than in normotension and the sensitivity to loop diuretics was threefold less. Intracellular [K] and the Na, K and Cl permeability ratios and electrogenic Na pump activity were assessed electrophysiologically in normotension and hypertension. [K]<sub>i<\/sub> was lower in hypertension (173\u00a0m<i>M<\/i>) than normotension (198\u00a0m<i>M<\/i>) but the other parameters (P<sub>Na\/Cl<\/sub> = 0.14, P<sub>Cl<\/sub>\/P<sub>K<\/sub> = 0.19 and electrogenic pump = \u20138.3\u00a0mV in normotension) were not significantly different. Ionic permeabilities to Na, K and Cl were significantly lower in hypertension than normotension. Plasma [Na], but not [K], was higher in hypertension than normotension. The conclusion is that increased activation of (Na+K+Cl) cotransport in hypertension plays a major role in the elevation of [Cl]<sub>i<\/sub> and depolarisation of the membrane potential in vascular smooth muscle in DOCA-salt hypertension. The role of (Na+K+Cl) cotransport in vascular smooth muscle in this model of hypertension is discussed in relation to [Cl]<sub>i<\/sub>, depolarisation of the membrane potential and contraction and in relation to cell growth.<\/jats:p>","DOI":"10.1159\/000025692","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T09:24:25Z","timestamp":1050398665000},"page":"492-501","source":"Crossref","is-referenced-by-count":21,"title":["Increased (Na+K+Cl) Cotransport in Rat Arterial Smooth Muscle in Deoxycorticosterone (DOCA)\/Salt-Induced Hypertension"],"prefix":"10.1159","volume":"36","author":[{"given":"R.A.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.R.","family":"Chipperfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.P.L.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.A.","family":"Harper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,12,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1993.1029"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1834"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.51.1.443"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(81)90150-3"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0895-7061(95)00074-Y"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050020"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900116"}],"container-title":["Journal of Vascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/25692","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/25692","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:50:20Z","timestamp":1745466620000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000025692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999]]},"references-count":7,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1999,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000025692","archive":["Portico"],"relation":{},"ISSN":["1018-1172","1423-0135"],"issn-type":[{"type":"print","value":"1018-1172"},{"type":"electronic","value":"1423-0135"}],"subject":[],"published":{"date-parts":[[1999]]}}}