{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:11:14Z","timestamp":1745554274596,"version":"3.40.4"},"reference-count":6,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[1999,10,15]],"date-time":"1999-10-15T00:00:00Z","timestamp":939945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,10,15]],"date-time":"1999-10-15T00:00:00Z","timestamp":939945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Blood Press Res"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>The signaling pathways involved in the regulation of glucocorticoid on Pi uptake were examined in primary cultured rabbit renal proximal tubule cells (PTCs). Dexamethasone (DEX, 10&lt;sup&gt;\u20139&lt;\/sup&gt; &lt;i&gt;M&lt;\/i&gt;) inhibited Pi uptake, although aldosterone, a mineralocorticoid, did not affect Pi uptake. Its effect was due to a 23% decrease in the V&lt;sub&gt;max&lt;\/sub&gt; value. DEX\u2013induced inhibition of Pi uptake was prevented by actinomycin D, cycloheximide, and the glucocorticoid receptor antagonists, progesterone and cortexolone. SQ 22536 (adenylate cyclase inhibitor) and the myristoylated protein kinase A inhibitor amide 14\u201322 (PKI) did not block the DEX\u2013induced inhibition of Pi uptake. Indeed, DEX did not affect cAMP production. However, neomycin and U 73122 (PLC inhibitors), staurosporine and bisindolylmaleimide I (PKC inhibitors) blocked the DEX\u2013induced inhibition of Pi uptake. In addition, DEX increased the membrane\u2013bound PKC activity from 2.82\u00b10.21 to 4.16\u00b10.34 pmol\/mg protein\/min. These findings demonstrate that glucocorticoid inhibits Pi uptake and its effect is genomic and receptor\u2013mediated and the activation of the PLC\/PKC pathway is involved in its effect on the PTCs.<\/jats:p>","DOI":"10.1159\/000025947","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T09:24:25Z","timestamp":1050398665000},"page":"1-9","source":"Crossref","is-referenced-by-count":8,"title":["Regulatory Mechanisms of Na\/Pi Cotransporter by Glucocorticoid in Renal Proximal Tubule Cells: Involvement of cAMP and PKC"],"prefix":"10.1159","volume":"23","author":[{"given":"Ho Jae","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae Hoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Hyun","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,10,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19970615)65:4<492::AID-JCB5>3.0.CO;2-J"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199901)178:1<35::AID-JCP5>3.3.CO;2-G"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000025915"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.95.1.118"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1999.0720380.x"}],"container-title":["Kidney and Blood Pressure Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/25947","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/25947","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:52:46Z","timestamp":1745466766000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000025947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,10,15]]},"references-count":6,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2000,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000025947","archive":["Portico"],"relation":{},"ISSN":["1420-4096","1423-0143"],"issn-type":[{"type":"print","value":"1420-4096"},{"type":"electronic","value":"1423-0143"}],"subject":[],"published":{"date-parts":[[1999,10,15]]}}}