{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:50:24Z","timestamp":1762159824850,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroimmunomodulation"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>Chronic neurodegeneration in the brains of Alzheimer\u2019s disease (AD) patients may be mediated, at least in part, by the ability of amyloid beta (A\u03b2) to exacerbate inflammatory pathways in a conformation-dependent manner. In this regard, we previously reported that the A\u03b2-peptide-mediated potentiation of inflammatory cytokine secretion from interleukin-1\u03b2 (IL-1\u03b2)-stimulated human astrocytoma cells was conformation dependent. Other amyloidogenic peptides, such as human amylin, which display similar conformation-dependent neurotoxic effects, may also elicit inflammatory cytokine secretion from glial cells. To test this hypothesis, we compared human and rat amylin for the effects on cytokine production in U-373 MG human astrocytoma cells. Human amylin alone stimulated U-373 MG cells to secrete IL-6 and IL-8 in a concentration-dependent manner with maximum effects seen at 10\u201325\u00a0\u03bc&lt;i&gt;M&lt;\/i&gt; peptide. In addition, human amylin markedly potentiated IL-1\u03b2-stimulated cytokine production with a similar concentration dependence. In contrast, nonamyloidogenic rat amylin modestly stimulated cytokine secretion, either alone or combined with IL-1\u03b2. Aging human amylin resulted in diminished cytokine secretion, probably due to the formation of large, less active aggregates. In agreement with our previous studies using A\u03b2, extracellular Ca&lt;sup&gt;2+&lt;\/sup&gt; was necessary for human amylin stimulation of cytokine secretion. Our data suggest that amyloidogenic peptides promote cytokine secretion through similar \u03b2-sheeted secondary-structure- and extracellular-Ca&lt;sup&gt;2+&lt;\/sup&gt;-dependent mechanisms.<\/jats:p>","DOI":"10.1159\/000026432","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T09:24:25Z","timestamp":1050398665000},"page":"147-152","source":"Crossref","is-referenced-by-count":16,"title":["Human Amylin Stimulates Inflammatory Cytokine Secretion from Human Glioma Cells"],"prefix":"10.1159","volume":"7","author":[{"given":"Bruce D.","family":"Gitter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura M.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Carlson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick C.","family":"May","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,4,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(91)90609-V"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.10.110194.002105"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5300.630"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0197-4580(92)90065-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(93)90009-B"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.9.4772"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/368756a0"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2574"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(91)90038-Q"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)90380-R"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91698-R"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(96)00156-4"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(94)90134-1"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0197-4580(92)90064-5"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9136(199706)14:2+<S9::AID-DIA397>3.3.CO;2-4"}],"container-title":["Neuroimmunomodulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/26432","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/26432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:57:19Z","timestamp":1745467039000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000026432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2000,4,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000026432","archive":["Portico"],"relation":{},"ISSN":["1021-7401","1423-0216"],"issn-type":[{"type":"print","value":"1021-7401"},{"type":"electronic","value":"1423-0216"}],"subject":[],"published":{"date-parts":[[2000]]}}}