{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:48:44Z","timestamp":1761742124069,"version":"3.40.4"},"reference-count":4,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[1999]]},"abstract":"<jats:p>The effects of repetitive field stimulation (model of hyperrelexia) on the responses of isolated strips of rabbit urinary bladder to FS and carbachol were evaluated under a variety of incubation conditions. Compared to control conditions, 2\u00a0h of repetitive FS in normal, oxygenated Tyrode\u2019s solution followed by incubation for 1\u00a0h with no stimulation resulted in a 50% decrease in contractile response to FS and a 30% decrease in the response to carbachol. Incubation in the absence of O&lt;sub&gt;2&lt;\/sub&gt; and glucose was used as an in vitro model for ischemia. Repetitive stimulation during in vitro ischemia resulted in a significantly greater decrease in the contractile responses to FS and carbachol than did in vitro ischemia without repetitive stimulation. The magnitude of contractile dysfunctions in response to both stimuli were significantly reduced in the presence of EGTA (calcium chelator), diltiazem (calcium channel blocker) or pincidil (potassium channel opener). Incubation with thapsigargin (SR calcium uptake inhibitor) + ryanodine (SR calcium storage inhibitor) had no effect. The results of these studies indicate that inhibition of Ca&lt;sup&gt;2+&lt;\/sup&gt; entry reduces the contractile dysfunctions induced by repetitive stimulation in the presence of in vitro ischemia. Inhibition of Ca&lt;sup&gt;2+&lt;\/sup&gt;&lt;sub&gt;i&lt;\/sub&gt; storage and release had no significant effect on the magnitude of contractile dysfunctions induced by repetitive stimulation an in vitro ischemia.<\/jats:p>","DOI":"10.1159\/000028273","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T09:24:25Z","timestamp":1050398665000},"page":"113-119","source":"Crossref","is-referenced-by-count":13,"title":["Correlation of EGTA and Calcium- Blocking Agents on the Response of the Bladder to in vitro Ischemia"],"prefix":"10.1159","volume":"58","author":[{"given":"Robert M.","family":"Levin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robbert","family":"Leggett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Whitbeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Horan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,2,5]]},"reference":[{"doi-asserted-by":"publisher","key":"ref1","DOI":"10.1002\/(SICI)1520-6777(1996)15:1<71::AID-NAU7>3.0.CO;2-C"},{"doi-asserted-by":"publisher","key":"ref2","DOI":"10.1159\/000028235"},{"doi-asserted-by":"publisher","key":"ref3","DOI":"10.1016\/0306-3623(86)90302-2"},{"doi-asserted-by":"publisher","key":"ref4","DOI":"10.1016\/0306-3623(88)90008-0"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/28273","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/28273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:15:08Z","timestamp":1745468108000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000028273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999]]},"references-count":4,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1999,3,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000028273","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"type":"print","value":"0031-7012"},{"type":"electronic","value":"1423-0313"}],"subject":[],"published":{"date-parts":[[1999]]}}}