{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:19:26Z","timestamp":1759331966539,"version":"3.40.4"},"reference-count":22,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>Human involucrin (hINV) is assembled into cornified structures via formation of transglutaminase (TG)-dependent interprotein \u03b5-(\u03b3-glutamyl)lysine bonds. The hINV sequence includes 150 glutamine residues that could function as potential sites of cross-link formation. The present studies were designed to evaluate the extent to which hINV can function as a TG substrate under optimal conditions and in the absence of other substrates. Incubation of hINV with TG results in formation of 4\u20135 isopeptide bonds per hINV molecule. When the small amine donor &lt;sup&gt;14&lt;\/sup&gt;C-putrescine is included in the reaction, 48 Q residues are labled. Isotope distribution and sequence analysis suggests that the &lt;sup&gt;14&lt;\/sup&gt;C-putrescine-labeled sites are located throughout the protein. Our present results show that many hINV Q residues can be utilized for cross-link formation, and that hINV can be cross-linked at very high cross-link densities. These results suggest that, in vivo, factors other than hINV structure limit the number of residues used for cross-link formation.<\/jats:p>","DOI":"10.1159\/000029905","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T17:26:45Z","timestamp":1050427605000},"page":"17-30","source":"Crossref","is-referenced-by-count":4,"title":["Transglutaminase Reactivity of Human Involucrin"],"prefix":"10.1159","volume":"13","author":[{"given":"Adam","family":"Lambert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meena","family":"Ekambaram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard L.","family":"Eckert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2000,2,12]]},"reference":[{"doi-asserted-by":"publisher","key":"ref1","DOI":"10.1111\/1523-1747.ep12472288"},{"doi-asserted-by":"publisher","key":"ref2","DOI":"10.1074\/jbc.270.30.17702"},{"doi-asserted-by":"publisher","key":"ref3","DOI":"10.1159\/000029830"},{"doi-asserted-by":"publisher","key":"ref4","DOI":"10.1074\/jbc.272.3.2021"},{"doi-asserted-by":"publisher","key":"ref5","DOI":"10.1074\/jbc.273.5.2721"},{"doi-asserted-by":"publisher","key":"ref6","DOI":"10.1074\/jbc.272.18.12035"},{"doi-asserted-by":"publisher","key":"ref7","DOI":"10.1074\/jbc.271.31.18817"},{"doi-asserted-by":"publisher","key":"ref8","DOI":"10.1074\/jbc.272.14.9410"},{"doi-asserted-by":"publisher","key":"ref9","DOI":"10.1074\/jbc.270.16.9590"},{"doi-asserted-by":"publisher","key":"ref10","DOI":"10.1074\/jbc.272.36.22736"},{"doi-asserted-by":"publisher","key":"ref11","DOI":"10.1016\/0014-5793(93)81772-R"},{"doi-asserted-by":"publisher","key":"ref12","DOI":"10.1074\/jbc.273.13.7604"},{"doi-asserted-by":"publisher","key":"ref13","DOI":"10.1038\/nm0698-713"},{"doi-asserted-by":"publisher","key":"ref14","DOI":"10.1073\/pnas.94.23.12604"},{"doi-asserted-by":"publisher","key":"ref15","DOI":"10.1074\/jbc.271.27.15922"},{"doi-asserted-by":"publisher","key":"ref16","DOI":"10.1074\/jbc.272.33.20577"},{"doi-asserted-by":"publisher","key":"ref17","DOI":"10.1083\/jcb.136.5.1151"},{"doi-asserted-by":"publisher","key":"ref18","DOI":"10.1046\/j.1432-1327.1998.2520216.x"},{"doi-asserted-by":"publisher","key":"ref19","DOI":"10.1074\/jbc.270.39.22855"},{"doi-asserted-by":"publisher","key":"ref20","DOI":"10.1006\/bbrc.1994.1416"},{"doi-asserted-by":"publisher","key":"ref21","DOI":"10.1073\/pnas.93.25.14580"},{"doi-asserted-by":"publisher","key":"ref22","DOI":"10.1074\/jbc.274.16.11013"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/29905","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/29905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:28:21Z","timestamp":1745468901000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000029905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2000,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000029905","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"type":"print","value":"1660-5527"},{"type":"electronic","value":"1660-5535"}],"subject":[],"published":{"date-parts":[[2000]]}}}