{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:31:30Z","timestamp":1787589090831,"version":"build-2736575974"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,3,25]],"date-time":"2003-03-25T00:00:00Z","timestamp":1048550400000},"content-version":"vor","delay-in-days":874,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p><jats:bold>Abstract<\/jats:bold>  <jats:bold>\n            <jats:italic>Background:<\/jats:italic>\n          <\/jats:bold> TSEN (MNS33) is a low\u2010incidence antigen in the MNS blood group system encoded by hybrid glycophorin genes. TSEN is expressed by a unique amino acid sequence that results from the junction of GPA<jats:sup>58<\/jats:sup> to GPB<jats:sup>27<\/jats:sup>, if the GPB carries S antigen. Until this study, only one example of anti\u2010TSEN had been found. Antibody screening red blood cells (RBCs) positive for both S and s (ref. No. C873) reacted with four patient sera. Initially, the RBCs had been typed as S+s+, but later were typed as S\u2010s+ in another laboratory. Two other RBC samples, one from a volunteer blood donor (D.L.), the other from a patient whose serum contained anti\u2010En<jats:sup>a<\/jats:sup>FR (J.S.), also gave anomalous results when tested with anti\u2010S. We sus pected the presence of TSEN\u2010positive hybrids on all three RBC samples. <jats:bold><jats:italic>Materials and Methods:<\/jats:italic><\/jats:bold> Reactive sera (O.B., E.C., S.K., R.F.) were tested against RBCs with normal MNS phenotypes and with TSEN\u2010positive RBCs. The RBCs of D.L., J.S. and C873 were tested with anti\u2010S whose reactivity with S+s+ TSEN+RBCs had been established previously, and with the original example of anti\u2010TSEN. Immunoblotting was performed on the C873, D.L. and J.S. RBC membranes using a monoclonal antibody to an epitope common to both glycophorin A and glycophorin B. <jats:bold><jats:italic>Results:<\/jats:italic><\/jats:bold> The sera from O.B., E.C., S.K. and R.F. were strongly reactive on the indirect antiglobulin test with TSEN+ RBCs. The RBCs of C873, D.L. and J.S. were typed as TSEN+. Immunoblotting pattern of D.L. and C873 were consistent with TSEN heterozygotes, while that of J.S. was consistent with a TSEN homozygote. <jats:bold><jats:italic>Conclusions:<\/jats:italic><\/jats:bold> Based on the estimated number of screening events with C873 RBCs, the incidence of anti\u2010TSEN is approximately 1 in 20,000 sera. The antibody is found in patients with and without documented exposure to allogeneic RBCs. All known examples of anti\u2010TSEN are lgG, but their clinical significance is not known.<\/jats:p>","DOI":"10.1046\/j.1423-0410.2000.7930175.x","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T22:53:17Z","timestamp":1104360797000},"page":"175-179","source":"Crossref","is-referenced-by-count":4,"title":["Four Examples of Anti\u2010TSEN and Three of TSEN\u2010Positive Erythrocytes"],"prefix":"10.1111","volume":"79","author":[{"given":"Jill R.","family":"Story","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwenn","family":"Lindsay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Rolih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuncion","family":"Co","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Rodberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion E.","family":"Reid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,25]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1992.tb02498.x"},{"key":"e_1_2_1_3_2","first-page":"17486","article-title":"Amino acid sequence of an \u03b1\u2010\u03b3\u2010glycophorin hybrid","volume":"264","author":"Johe KK","year":"1989","journal-title":"A structure reciprocal to Sta\u03b3-\u03b1-glycophorin hybrid. 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