{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:56:04Z","timestamp":1760644564208,"version":"3.40.4"},"reference-count":5,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[1999]]},"abstract":"Marginal-zone B cells of the mucosa-associated lymphoid tissue (MALT) are the normal counterpart of the neoplastic cells in MALT lymphoma. In both cases these lymphocytes express surface immunoglobulins, but are negative when stained for B cell associated antigens like CD10 and CD23. Furthermore, the B cell gene rearrangement has been found in <i>Helicobacter pylori<\/i> associated chronic gastritis and in extranodal type of marginal-zone lymphoma. The aim of this study was to quantify the number of IgM-, CD10-, and CD23-positive lymphocytes in patients with type B gastritis and to compare the results with the antigen profile of mononuclear cells in patients with gastritis not associated with <i>H.\u00a0pylori<\/i>. Additionally, the immunoglobulin heavy-chain (IgH) gene rearrangement in <i>H.\u00a0pylori<\/i> positive and <i>H.\u00a0pylori<\/i> negative gastritis was studied. From 23 patients with a positive urease test and\/or histologically proven <i>H. pylori<\/i> infection and chronic gastritis and from 22 patients with <i>H.\u00a0pylori<\/i> negative chronic gastritis mucosa biopsy specimens were taken. Single-cell suspensions were obtained following enzymatic digestion. For immunocytochemistry, an alkaline phosphatase-antialkaline phosphatase method was applied. IgH gene rearrangement in formalin-fixed, paraffin-embedded specimens was determined by polymerase chain reaction in 11 patients with chronic gastritis. An increase in mu-positive plasma cells and B lymphocytes was detected in patients with <i>H.\u00a0pylori<\/i> positive gastritis as compared with patients with <i>H.\u00a0pylori<\/i> negative gastritis (10.0 vs. 3.9%, p < 0.001, and 4.3 vs. 1.6%, p < 0.01, respectively). In both groups, the proportion of CD10- and CD23-positive lymphocytes was <1%. IgH gene rearrangement was not restricted to type\u00a0B gastritis; single bands were also present in 3 of 7 patients with <i>H.\u00a0pylori<\/i> negative chronic gastritis. Our finding of IgH gene rearrangement in some of the patients with <i>H.\u00a0pylori<\/i> negative chronic gastritis indicates that additional factors may be critical for these genotypical changes and for the pathogenesis of gastric MALT lymphoma.<\/jats:p>","DOI":"10.1159\/000040974","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T15:42:49Z","timestamp":1050421369000},"page":"77-82","source":"Crossref","is-referenced-by-count":3,"title":["Antigenic Phenotyping of Lymphoid Cells and B Cell Gene Rearrangement in Type B Gastritis and in Gastritis Not Associated with <i>Helicobacter pylori<\/i> Colonization"],"prefix":"10.1159","volume":"102","author":[{"given":"A.","family":"Fritscher-Ravens","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Petrasch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Tiemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Wacker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"D\u00f6rr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Kemmeries","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Hilgers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Reinacher-Schick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Kunstmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Schmiegel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,10,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92035-Z"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199609)180:1<26::AID-PATH681>3.3.CO;2-O"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0301-4622(87)80026-1"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301803"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/40974","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/40974","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:35:21Z","timestamp":1745469321000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000040974"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999]]},"references-count":5,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1999,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000040974","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"type":"print","value":"0001-5792"},{"type":"electronic","value":"1421-9662"}],"subject":[],"published":{"date-parts":[[1999]]}}}