{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T19:04:24Z","timestamp":1775675064603,"version":"3.50.1"},"reference-count":3,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2001]]},"abstract":"<jats:p>&lt;i&gt;Background\/Aim:&lt;\/i&gt; A lymphokine, the vascular permeability factor (VPF), which increases vascular permeability, has been characterized in minimal-change nephrotic syndrome (MCNS). Transforming growth factor-\u03b2 (TGF-\u03b2) is an immunosuppressive cytokine that inhibits proliferation, cytokine production, and cytotoxic activity of T cells and natural killer cells. We, therefore, investigated the effects of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; on the release of VPF by peripheral blood T cells from MCNS patients. The aim of our study was to determine the in vitro immunosuppressive capacity of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; in patients with MCNS. &lt;i&gt;Methods:&lt;\/i&gt; To further test the effect of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; on concanavalin A (Con A)-induced VPF release, normal and MCNS T cells were stimulated with 5\u00a0\u00b5g\/ml of Con A in the presence or absence of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt;, and the culture supernatants were tested for the presence of VPF by the method of Lagrue et al. The disease controls included 16 patients with IgA nephropathy. &lt;i&gt;Results:&lt;\/i&gt; Significantly increased spontaneous and Con A-stimulated secretion of VPF was detected in T-cell cultures of MCNS patients with the nephrotic syndrome as compared with those of normal controls. Addition of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; to these cultures inhibited the release of VPF in a dose-dependent manner. The effect of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; on the release of VPF was specific, since a reversion was obtained with a neutralizing monoclonal antibody to human TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt;. &lt;i&gt;Conclusion:&lt;\/i&gt; Together, our data demonstrate that TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; antagonizes the ability of T cells to release VPF, and suggest a role of TGF-\u03b2&lt;sub&gt;1&lt;\/sub&gt; in the pathophysiology of VPF in vitro.<\/jats:p>","DOI":"10.1159\/000045898","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:15Z","timestamp":1050433575000},"page":"111-117","source":"Crossref","is-referenced-by-count":17,"title":["Transforming Growth Factor-\u03b2&lt;sub&gt;1&lt;\/sub&gt; Inhibits Vascular Permeability Factor Release by T Cells in Normal Subjects and in Patients with Minimal-Change Nephrotic Syndrome"],"prefix":"10.1159","volume":"87","author":[{"given":"Koichi","family":"Matsumoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuo","family":"Kanmatsuse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,2,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000013420"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(89)90136-9"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/332260a0"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/45898","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/45898","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:48:33Z","timestamp":1745470113000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000045898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":3,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2001,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000045898","archive":["Portico"],"relation":{},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001]]}}}