{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:14:15Z","timestamp":1745554455708,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"published-print":{"date-parts":[[2001]]},"abstract":"<jats:p>It has been shown that chronic hemodialysis modifies, to some extent, the normal immune response by both T and B lymphocytes elicited by antigenic stimulation, e.g. by impairing the T-cell-dependent response after vaccination. A new technique, i.e. flow cytometry, enables to assess intracytoplasmically, at the single cell level, the production of a given cytokine. By using it, we studied in healthy volunteers (HV) and in chronic hemodialysis (CHD) patients, with respect to their hepatitis C virus (HCV) status, the production by the T lymphocytes of type 1, and type 2 cytokines. We studied the following cytokines (CK): IL-2, IL-4, IL-5, IL-6, IL-10, IFN-\u03b3 and TNF-\u03b1 in the T-cell lymphocytes (whole, CD4+ and CD8+). There were 13 HV and 59 CHD patients (36 HCV(\u2013) and 23 HCV(+)). Amongst the latter, there were 32 men and 27 women, aged 59.5 \u00b1 2 years, undergoing CHD since 70 \u00b1 9.4 months. We found that: (1) the total number of lymphocytes as well as those expressing CD3, CD4, or CD19 were significantly decreased in CHD patients as compared to those from HV; (2) the total number of lymphocytes as well as their different subsets were similar in HCV(+) and in HCV(\u2013) CHD patients; (3) the frequency of T-cell-expressing IL-5 or IL-10 was always low (&lt;1%) in both HV and CHD groups; (4) overall in CHD patients, the mean percentages of T lymphocytes expressing IL-2, IL-4, IFN-\u03b3 or TNF-\u03b1 were respectively 31 \u00b1 13, 2.5 \u00b1 1.3, 28 \u00b1 12 and 34 \u00b1 11% and were not statistically different between HCV(+) and HCV(\u2013) patients; (5) IL-2 was mainly produced by CD4+ T cells, whereas IFN-\u03b3 was produced by CD8+ T cells, in both HV and CHD groups, and (6) the lymphocytes of CHD patients produced significantly more IL-2 and IL-4 than those from HV, suggesting an activation of their T lymphocytes. We conclude that using the cytokine flow cytometry assay, our study demonstrated that in HCV(+) CHD patients, as opposed to what has been described for HCV(+) patients with normal renal function, there was no impairment in the production of type 1 cytokines by peripheral blood mononuclear cells when compared to HCV(\u2013) CHD patients. Conversely to HV, T lymphocytes from CHD patients are activated.<\/jats:p>","DOI":"10.1159\/000046247","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"194-199","source":"Crossref","is-referenced-by-count":5,"title":["Lack of Effect of Chronic Hepatitis C Virus Infection on T-Cell Cytokine Production in Chronic Hemodialysis Patients"],"prefix":"10.1159","volume":"21","author":[{"given":"Lionel","family":"Rostaing","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean S\u00e9bastien","family":"Borde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Hasle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Bories","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asma","family":"Allal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Abbal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Durand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,6,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199906)58:2<139::AID-JMV7>3.3.CO;2-Z"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90158-4"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19990401)35:4<318::AID-CYTO4>3.3.CO;2-W"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0012.1999.130506.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80093-2"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)06088-1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5390.938"}],"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/46247","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/46247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:52:53Z","timestamp":1745470373000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000046247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":7,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2001,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000046247","archive":["Portico"],"relation":{},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"type":"print","value":"0250-8095"},{"type":"electronic","value":"1421-9670"}],"subject":[],"published":{"date-parts":[[2001]]}}}