{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T15:32:58Z","timestamp":1767108778637,"version":"3.40.4"},"reference-count":3,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2001]]},"abstract":"Sepsis-induced suppression in T-cell proliferation follows deranged Ca<sup>2+<\/sup> signaling in adult rats. In preliminary studies, we observed suppression in T-cell proliferation in septic neonatal rats as well. In this study, we assessed splenic T-cell cytosolic Ca<sup>2+<\/sup> concentration, [Ca<sup>2+<\/sup>]<sub>i<\/sub>, as its elevation plays an important role in T-cell proliferation. Also, we investigated the role of PGE<sub>2<\/sub> in sepsis-related changes in T-cell [Ca<sup>2+<\/sup>]<sub>i<\/sub> in animals pretreated with cyclooxygenase-1 (COX-1) inhibitor (resveratrol) and cyclooxygenase-2 (COX-2) inhibitor (NS-398). Sepsis was induced in 15-day-old rat pups by intraperitoneal implantation of fecal pellets containing <i>Escherichia coli<\/i> and <i>Bacteroides fragilis<\/i>. The sham group consisted of pups implanted with sterile fecal pellets. Septic and sham pups were sacrificed 24 h after implantation and their spleens were removed. The spleens from sham and septic pups, along with spleens from unoperated control pups, were processed for single cell suspensions, and T cells were isolated using nylon wool columns. Fura-2 fluorophotometry was employed for the measurement of [Ca<sup>2+<\/sup>]<sub>i<\/sub> (in n<i>M<\/i> units) in T cells stimulated with concanavalin A (ConA). Our results show that ConA-mediated T-cell [Ca<sup>2+<\/sup>]<sub>i<\/sub> response is significantly suppressed in septic neonatal rats. Pretreatment of pups with COX-2, but not COX-1 inhibitor, prevented the decrease in the [Ca<sup>2+<\/sup>]<sub>i<\/sub> response. These findings suggest that PGE<sub>2<\/sub> might induce the attenuation in T-cell Ca<sup>2+<\/sup> signaling during sepsis in neonatal rats.<\/jats:p>","DOI":"10.1159\/000047160","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T19:06:15Z","timestamp":1050433575000},"page":"300-304","source":"Crossref","is-referenced-by-count":1,"title":["Sepsis-Induced Alteration in T-Cell Ca<sup>2+<\/sup> Signaling in Neonatal Rats"],"prefix":"10.1159","volume":"80","author":[{"given":"Mohammad H.","family":"Alattar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thyyar M.","family":"Ravindranath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mashkoor A.","family":"Choudhry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan K.","family":"Muraskas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahla Y.","family":"Namak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ousama","family":"Dallal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed M.","family":"Sayeed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199704000-00007"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199404213301602"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(93)90218-M"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/47160","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/47160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:49:37Z","timestamp":1745466577000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000047160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":3,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2001,10,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000047160","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2001]]}}}