{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:12:33Z","timestamp":1745554353956,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Immunogenet"],"published-print":{"date-parts":[[2001]]},"abstract":"<jats:p>We present evidence here that the proinflammatory cytokine, interleukin-1\u03b2 (IL-1\u03b2) stimulates a significant increase in protein kinase C (PKC)-&amp;#917; and PKC-\u03b4 protein levels and increases PKC-&amp;#917;, but not PKC-\u03b4, transcripts in EL4 thymoma cells. Incubation of EL4 cells with IL-1\u03b2 induced protein synthesis of PKC-&amp;#917; (6-fold increase) by 7\u00a0h and had a biphasic effect on PKC-\u03b4 levels with peaks at 4\u00a0h (2-fold increase) and 24\u00a0h (4-fold increase). At the level of mRNA, PKC-&amp;#917;, but not PKC-\u03b4 levels, were induced after incubation of EL4 cells with IL-1\u03b2. The signalling mechanisms utilized by IL-1\u03b2 to induce the synthesis of these PKC isoforms were investigated. Two phosphatidylinositol (PI) 3-kinase-specific inhibitors, wortmannin and LY294002, inhibited IL-1\u03b2-induced synthesis of PKC-&amp;#917;. However, the PI 3-kinase inhibitors had little effect on the IL-1\u03b2-induced synthesis of PKC-\u03b4 in these cells. Our results indicate that IL-1\u03b2 induced both PKC-\u03b4 and PKC-&amp;#917; expression over different time periods. Furthermore, our evidence suggests that IL-1\u03b2 induction of PKC-&amp;#917;, but not PKC-\u03b4, may occur via the PI 3-kinase pathway.<\/jats:p>","DOI":"10.1159\/000049193","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"135-142","source":"Crossref","is-referenced-by-count":2,"title":["Interleukin-1\u03b2 Induced Synthesis of Protein Kinase C-\u03b4 and Protein Kinase C-\u03b5 in EL4 Thymoma Cells: Possible Involvement of Phosphatidylinositol 3-Kinase"],"prefix":"10.1159","volume":"18","author":[{"given":"Claire L.","family":"Varley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice A.","family":"Royds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry L.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pauline R.M.","family":"Dobson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,9,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.28.16514"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.46.29167"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90534-0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(90)90074-7"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1987.9999"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.43.25352"}],"container-title":["Experimental and Clinical Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/49193","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/49193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:12:25Z","timestamp":1745467945000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000049193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":7,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2001,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000049193","archive":["Portico"],"relation":{},"ISSN":["0254-9670","1421-9948"],"issn-type":[{"type":"print","value":"0254-9670"},{"type":"electronic","value":"1421-9948"}],"subject":[],"published":{"date-parts":[[2001]]}}}