{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T09:46:16Z","timestamp":1760607976914,"version":"3.40.4"},"reference-count":11,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2001]]},"abstract":"<i>Background:<\/i> The major allergen of the dust mite<i> Lepidoglyphus destructor<\/i>, Lep d 2, has been produced as a recombinant allergen (rLep d 2) with IgE reactivity both in vivo and in vitro. A modified form of rLep d 2 (rLep d 2.6Cys) obtained by site-directed mutagenesis has been shown to have a reduced IgE reactivity in vitro. In this study we have compared the ability of rLep d 2 and rLep d 2.6Cys to elicit positive skin prick tests and cellular responses among<i> L. destructor<\/i>-sensitized subjects. <i>Methods:<\/i> Seventeen subjects were skin prick-tested with rLep d 2, rLep d 2.6Cys, histamine and negative controls and 17\u201320 h later skin biopsy specimens were taken from the skin prick-tested sites. The biopsy specimens were stained immunohistochemically for EG2+, CD3+, CD1a+, mast cell tryptase+, and IgE+ cells. Dermal cell infiltrates were judged in hematoxylin and eosin staining. Total IgE and allergen-specific IgE were determined by CAP-RAST. <i>Results:<\/i> Compared to rLep d 2, rLep d 2.6Cys induced significantly smaller and fewer skin prick test reactions (p < 0.001) and dermal cell infiltrates (p < 0.05). Further, rLep d 2.6Cys induced fewer EG2+ cells (p < 0.001) but more tryptase+ cells (p < 0.05) than rLep d 2. A positive RAST to rLep d 2 was obtained for 88.2% of the subjects, while only 35.2% displayed a positive RAST to rLep d 2.6Cys. <i>Conclusion:<\/i> This study demonstrates that rLep d 2.6Cys is less able to evoke IgE-mediated reactions and cellular responses, as measured both in skin and in serum, than rLep d 2. In the future this hypoallergenic derivative may be a promising candidate molecule for immunotherapy of <i>L. destructor<\/i>-allergic patients.<\/jats:p>","DOI":"10.1159\/000049493","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"41-49","source":"Crossref","is-referenced-by-count":8,"title":["A Hypoallergenic Derivative of the Major Allergen of the Dust Mite <i>Lepidoglyphus destructor<\/i>, Lep d 2.6Cys, Induces Less IgE Reactivity and Cellular Response in the Skin than Recombinant Lep d 2"],"prefix":"10.1159","volume":"126","author":[{"given":"Marianne","family":"Kronqvist","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Whitley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna","family":"Olsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guro","family":"Gafvelin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annika","family":"Scheynius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"van Hage-Hamsten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.109832"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000024352"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2000.00120.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00452.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.1999.00996.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.1.356"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.112264"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000024218"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00418.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.106924"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.106884"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/49493","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/49493","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:16:01Z","timestamp":1745468161000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000049493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2001,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000049493","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2001]]}}}