{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:38:22Z","timestamp":1790318302605,"version":"4.1.0"},"reference-count":17,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2001]]},"abstract":"<jats:p>Skin-homing T cells are defined by the expression of the cutaneous lymphocyte-associated antigen (CLA) which enables the cells to selectively bind to vascular endothelial E-selectin close to sites of cutaneous inflammation, an initial step in the effective extravasation from blood into the inflamed tissue. Essentially all CLA on T cells decorates the backbone of the P-selectin glycoprotein ligand-1 (PSGL-1). In this study we show that human peripheral blood B cells (PBBC) and tonsillar B cells (TBC) do not display PSGL-1 in fluorescence-activated cell sorter analysis using different murine monoclonal antibodies and polyclonal rabbit anti-PSGL-1 antiserum. A significant population of TBC, however, expresses a HECA-452-reactive epitope. These cells represent nonactivated IgM&lt;sup&gt;+&lt;\/sup&gt;\/IgG&lt;sup&gt;\u2013&lt;\/sup&gt; mature B lymphocytes. Up to 50% of the TBC in a given preparation strongly bind to E- and up to 79% to P-selectin. The shear stress resistance in a parallel-plate flow chamber system was high. Neuraminidase treatment of TBC totally and O-sialoglycoprotein endopeptidase partially diminished HECA-452 reactivity and reduced E- but not P-selectin ligand activities. Mocarhagin had no effect in the assays. The data suggest a different ligand for P-selectin and a distinct glycoprotein carrier for the E-selectin ligand as compared to T cells or other leukocytes. Adhesion to P-selectin, however, still required sulfation of the ligand for function. Western blots of TBC cell lysates detected a &gt;240-kD HECA-452-reactive material that was resistant to reducing conditions. Anti-PSGL-1 did not reveal immunoreactive material in these cell lysates. B cell activation did neither significantly change HECA positivity nor induce PSGL-1 expression. Cultured, activated TBC, however, maintained expression of the integrin \u03b14\u03b27. Human peripheral blood B cells had similar cell surface characteristics to TBC. Our observations suggest that several adhesion molecules may be involved in B cell homing which include CLA, the P-selectin ligand, and structures such as \u03b14\u03b27.<\/jats:p>","DOI":"10.1159\/000049497","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"78-90","source":"Crossref","is-referenced-by-count":5,"title":["Tonsillar B Cells Do Not Express PSGL-1, but a Significant Fraction Displays the Cutaneous Lymphocyte Antigen and Exhibits Effective E- and P-Selectin Ligand Activity"],"prefix":"10.1159","volume":"126","author":[{"given":"Dieter","family":"Armerding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Fuhlbrigge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. David","family":"Kieffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Kupper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.03031.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.6.1461"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(97)01040-X"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(94)90118-X"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/40166"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000024197"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.747"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.37.21966"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2799"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90279-8"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.45.26734"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.11.6342"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.19.11662"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80137-3"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.12.1769"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.1.263"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.381"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/49497","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/49497","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:16:04Z","timestamp":1745468164000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000049497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":17,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2001,9,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000049497","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001]]}}}