{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T21:22:21Z","timestamp":1770499341598,"version":"3.49.0"},"reference-count":4,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2002]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Human respiratory syncytial virus (RSV) infection in infancy and early childhood causes acute bronchiolitis and exacerbates bronchial asthma. Eosinophil infiltration may contribute to airway obstruction in RSV infection. &lt;i&gt;Objective:&lt;\/i&gt; We hypothesized that RSV affects eosinophil function. &lt;i&gt;Methods:&lt;\/i&gt; Eosinophil activation was evaluated by chemiluminescent detection of superoxide anion (O&lt;sup&gt;\u2013&lt;\/sup&gt;&lt;sub&gt;2&lt;\/sub&gt;) generation. Expression of CD11b on eosinophils was determined by flow cytometry. &lt;i&gt;Results:&lt;\/i&gt; Although RSV did not induce O&lt;sup&gt;\u2013&lt;\/sup&gt;&lt;sub&gt;2&lt;\/sub&gt; generation by resting eosinophils, RSV enhanced O&lt;sup&gt;\u2013&lt;\/sup&gt;&lt;sub&gt;2&lt;\/sub&gt; generation of eosinophils primed with platelet-activating factor (PAF). Enhancement was significantly inhibited by either continuous agitation to prevent eosinophil adhesion to test tube surfaces or by pretreating cells with anti-CD18 antibody, suggesting that the stimulatory effects of RSV on eosinophils depend on cell adhesion via \u03b2&lt;sub&gt;2&lt;\/sub&gt;-integrins. In fact, RSV enhanced PAF-induced CD11b expression by eosinophils. &lt;i&gt;Conclusion:&lt;\/i&gt; These findings suggest that RSV enhances eosinophil CD11b expression and O&lt;sup&gt;\u2013&lt;\/sup&gt;&lt;sub&gt;2&lt;\/sub&gt; generation induced by PAF. Thus, RSV infection may exacerbate airway inflammation by enhancing mediator release from eosinophils.<\/jats:p>","DOI":"10.1159\/000050086","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T10:10:37Z","timestamp":1050401437000},"page":"43-51","source":"Crossref","is-referenced-by-count":8,"title":["Respiratory Syncytial Virus Enhances the Expression of CD11b Molecules and the Generation of Superoxide Anion by Human Eosinophils Primed with Platelet-Activating Factor"],"prefix":"10.1159","volume":"45","author":[{"given":"Atsushi","family":"Tachibana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Nako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunihisa","family":"Kozawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Morikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2002,4,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)10321-5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.6.1489"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90721-S"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90054-X"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/50086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/50086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:19:47Z","timestamp":1745468387000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000050086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002]]},"references-count":4,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2002,2,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000050086","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"value":"0300-5526","type":"print"},{"value":"1423-0100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002]]}}}