{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:29:37Z","timestamp":1772641777933,"version":"3.50.1"},"reference-count":7,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2001]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Increased interleukin-5 (IL-5) levels have been reported in bronchoalveolar lavage fluid (BALF) from patients with acute eosinophilic pneumonia (AEP); however, it still remains to be determined whether IL-5 is responsible for the eosinophil accumulation in the lung. &lt;i&gt;Objective:&lt;\/i&gt; We examined the effect of antibodies against cytokines on eosinophil chemotaxis induced by BALF from AEP patients to identify factors responsible for eosinophil accumulation. &lt;i&gt;Methods:&lt;\/i&gt; We measured a series of specific cytokines, including IL-3, IL-4, IL-5, IL-6, IL-8, GM-CSF, RANTES, MCP-1, MIP-1\u03b1 and eotaxin, in the BALF from 4 patients with AEP. BALF from 4 patients with chronic eosinophilic pneumonia (CEP) and 13 patients with non-eosinophilic interstitial lung diseases (ILD) were examined as controls. The eosinophil chemotactic activity in the BALF was examined using tissue culture insert furnished with a polycarbonate membrane. &lt;i&gt;Results:&lt;\/i&gt; The total protein content in BALF from patients with AEP was extremely elevated. Even after standardization with protein concentration, IL-5 levels in AEP patients were significantly higher than those in CEP and ILD. IL-3 and chemokines were rather lower in the AEP group than in the CEP and ILD groups. In AEP BALF, anti-IL-5 neutralizing antibody significantly inhibited eosinophil chemotaxis. Antibodies against IL-3, GM-CSF, and IL-8 did not affect the eosinophil migration. &lt;i&gt;Conclusion:&lt;\/i&gt; These findings suggest that locally produced IL-5 plays an important role in eosinophil accumulation of AEP.<\/jats:p>","DOI":"10.1159\/000050532","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T12:11:24Z","timestamp":1050408684000},"page":"389-395","source":"Crossref","is-referenced-by-count":29,"title":["Increased Interleukin-5 Levels in Bronchoalveolar Lavage Fluid Is a Major Factor for Eosinophil Accumulation in Acute Eosinophilic Pneumonia"],"prefix":"10.1159","volume":"68","author":[{"given":"Yoshiaki","family":"Nakahara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichiro","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Fukuno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiro","family":"Kawashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Yatsunami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2001,7,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.1.219"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.3.881"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.2.587"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(89)90339-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.1.43"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(95)80008-7"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.10.1.295"}],"container-title":["Respiration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/50532","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/50532","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:56:04Z","timestamp":1745466964000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000050532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":7,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2001,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000050532","archive":["Portico"],"relation":{},"ISSN":["0025-7931","1423-0356"],"issn-type":[{"value":"0025-7931","type":"print"},{"value":"1423-0356","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001]]}}}