{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:49:03Z","timestamp":1790682543797,"version":"4.1.0"},"reference-count":6,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmologica"],"published-print":{"date-parts":[[2001]]},"abstract":"<i>Purpose:<\/i> To clarify the effect of bradykinin on cytosolic free calcium mobilization and cell proliferation in cultured bovine corneal endothelial cells (BCEC). <i>Methods:<\/i> The cytosolic free calcium concentration (Ca<sup>2+<\/sup>]<sub>i<\/sub>) was measured with the InCa<sup>TM<\/sup> Imaging System after the treatment of bradykinin (10<sup>\u201311<\/sup> to 10<sup>\u20137<\/sup>\u00a0<i>M<\/i>) alone or with the pretreatments of EGTA, bradykinin receptor (Bk<sub>1<\/sub> and Bk<sub>2<\/sub>) antagonists and an inhibition of phospholipase C (U-73122). Also, the effect of bradykinin on cell proliferation in BCEC was evaluated using cell counts. <i>Results:<\/i> In BCEC, [Ca<sup>2+<\/sup>]<sub>i<\/sub> in the resting state was 87 \u00b1 9 n<i>M<\/i>. Bradykinin induced an increment of [Ca<sup>2+<\/sup>]<sub>i<\/sub> in a concentration-dependent manner and its 50% effective concentration was approximately 5 \u00d7 10<sup>\u201311<\/sup>\u00a0<i>M<\/i>. A [Ca<sup>2+<\/sup>]<sub>i<\/sub> increment at 10<sup>\u20138<\/sup>\u00a0<i>M<\/i> bradykinin was inhibited with the pretreatment of EGTA, an extracellular calcium chelator. U-73122 (5 \u00d7 10<sup>\u20136<\/sup>\u00a0<i>M<\/i>) attenuated the bradykinin-induced [Ca<sup>2+<\/sup>]<sub>i<\/sub> increment. The pretreatment of HOE-140 (Bk<sub>2<\/sub> antagonist) almost attenuated the bradykinin (10<sup>\u20138<\/sup>\u00a0<i>M<\/i>)-induced [Ca<sup>2+<\/sup>]<sub>i<\/sub> increase, but des-Arg<sup>9<\/sup>-[Leu<sup>8<\/sup>]-bradykinin (Bk<sub>1<\/sub> antagonist) did not suppress it. To investigate the physiological effect of bradykinin, the effect of bradykinin on cell proliferation was studied. 10<sup>\u20138<\/sup>\u00a0<i>M<\/i> of bradykinin produced a significant increase in cell numbers. This mitogenic effect of bradykinin was inhibited by the Bk<sub>2<\/sub> antagonist. <i>Conclusions:<\/i> Bradykinin-induced stimulation of the signal transduction pathway in BCEC is coupled with the Bk<sub>2<\/sub> type receptor. Furthermore, bradykinin produces the mitogenic effect in BCEC.<\/jats:p>","DOI":"10.1159\/000050879","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T12:11:24Z","timestamp":1050408684000},"page":"303-308","source":"Crossref","is-referenced-by-count":17,"title":["Effect of Bradykinin on Cultured Bovine Corneal Endothelial Cells"],"prefix":"10.1159","volume":"215","author":[{"given":"Suk-Woo","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woon Kyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Jung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoung-Ah","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kweon-Haeng","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyoung Kyun","family":"Rha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Won","family":"Hahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2001,5,31]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.17.10.994.5242"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0162-3109(96)00025-2"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00256-6"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.82.181"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1835"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27788"}],"container-title":["Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/50879","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/50879","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T03:59:18Z","timestamp":1745467158000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000050879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001]]},"references-count":6,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2001,8,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000050879","archive":["Portico"],"relation":{},"ISSN":["0030-3755","1423-0267"],"issn-type":[{"value":"0030-3755","type":"print"},{"value":"1423-0267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001]]}}}