{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:50:56Z","timestamp":1774565456008,"version":"3.50.1"},"reference-count":5,"publisher":"S. Karger AG","issue":"Suppl. 1","license":[{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[1999,1,1]],"date-time":"1999-01-01T00:00:00Z","timestamp":915148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[1999]]},"abstract":"<jats:p>&lt;b&gt;Background:&lt;\/b&gt; Vascular endothelial growth factor (VEGF), a potent angiogenic factor and vasodilator, is strongly expressed by epidermal keratinocytes in many angiogenesis-dependent skin disorders. Retinoids may modulate VEGF in skin and this may be related to an effect on rosacea. &lt;b&gt;Aim:&lt;\/b&gt; To investigate the effect of retinaldehyde on VEGF production by human keratinocytes. &lt;b&gt;Methods:&lt;\/b&gt; The effects of different concentrations of retinoids (all-&lt;i&gt;trans&lt;\/i&gt;-retinal and all-&lt;i&gt;trans&lt;\/i&gt;-retinoic acid) on VEGF production by cultured human skin keratinocytes in both cell extracts and supernatants were determined. Expression of VEGF was analyzed by enzyme-linked immunosorbent assay (ELISA) and RT-PCR. &lt;b&gt;Results:&lt;\/b&gt; The amount of cell-associated and secreted VEGF strongly decreased with retinoid concentration (e.g. 48, 69% inhibition at 0.1 \u00b5&lt;i&gt;M&lt;\/i&gt; all-&lt;i&gt;trans&lt;\/i&gt;-retinal and -retinoic acid, respectively, in the supernatants). In parallel, approximately 25% inhibition of VEGF mRNA expression was obtained in the presence of 0.01 \u00b5&lt;i&gt;M&lt;\/i&gt; all-&lt;i&gt;trans&lt;\/i&gt;-retinal. &lt;b&gt;Conclusion:&lt;\/b&gt; The decrease in VEGF expression by keratinocytes on contact with retinoids may prevent skin neoangiogenesis in certain skin diseases.<\/jats:p>","DOI":"10.1159\/000051374","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T12:11:24Z","timestamp":1050408684000},"page":"25-27","source":"Crossref","is-referenced-by-count":26,"title":["Inhibitory Effects of Retinoids on Vascular Endothelial Growth Factor Production by Cultured Human Skin Keratinocytes"],"prefix":"10.1159","volume":"199","author":[{"given":"S.","family":"Lachgar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Charv\u00e9ron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Gall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.L.","family":"Bonaf\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[1999,8,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0195-27"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.5.1375"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90669-9"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00393.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.6.1535"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/51374","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/51374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:05:09Z","timestamp":1745467509000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000051374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999]]},"references-count":5,"journal-issue":{"issue":"Suppl. 1","published-online":{"date-parts":[[1999,7,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000051374","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999]]}}}